Preliminary Study: Characterisation of Antibody for rLipl32 Protein of Leptospira

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dc.contributor en-US
dc.creator ., Sumarningsih; Indonesian Research Center for Veterinary Science
dc.creator Tarigan, Simson; Indonesian Research Center for Veterinary Science
dc.date 2017-03-31
dc.date.accessioned 2018-06-04T07:56:32Z
dc.date.available 2018-06-04T07:56:32Z
dc.identifier http://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1460
dc.identifier 10.14334/Proc.Intsem.LPVT-2016-p.147-152
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/4918
dc.description Leptospirosis infection is commonly determined by positive response antibody in microaglutination test (MAT). The test requires panels of Leptospira from complete serovars and uses infectious Leptospira, which is subcultured weekly. Accordingly, other alternative serological tests are developed for MAT. One of them is ELISA using recombinant LipL32 protein (rLipL32). High sensitivity and specificity of ELISA rLipL32 for Leptospirosis detection in bovine and canine has been reported previously. In this study, we produced antibody to rLipL32 protein with high titre. Results on western blotting and ELISA showed anti-LipL32 antibody was highly binding to rLipL32 protein. In contrast, MAT results for LipL32 antibody was found to be negative response, indicating that titre antibody to LipL32 does not positively correlate to MAT. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Animal Science Research and Development (ICARD) en-US
dc.relation http://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1460/1345
dc.rights Copyright (c) 2017 Proceedings International Seminar on Livestock Production and Veterinary Technology en-US
dc.source Proceedings of International Seminar on Livestock Production and Veterinary Technology; 2016; 147-152 en-US
dc.subject Leptospira; Antibody Response; LipL32; MAT en-US
dc.title Preliminary Study: Characterisation of Antibody for rLipl32 Protein of Leptospira en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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