Preliminary Study: Characterisation of Antibody for rLipl32 Protein of Leptospira

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dc.contributoren-US
dc.creator., Sumarningsih; Indonesian Research Center for Veterinary Science
dc.creatorTarigan, Simson; Indonesian Research Center for Veterinary Science
dc.date2017-03-31
dc.date.accessioned2018-06-04T07:56:32Z
dc.date.available2018-06-04T07:56:32Z
dc.descriptionLeptospirosis infection is commonly determined by positive response antibody in microaglutination test (MAT). The test requires panels of Leptospira from complete serovars and uses infectious Leptospira, which is subcultured weekly. Accordingly, other alternative serological tests are developed for MAT. One of them is ELISA using recombinant LipL32 protein (rLipL32). High sensitivity and specificity of ELISA rLipL32 for Leptospirosis detection in bovine and canine has been reported previously. In this study, we produced antibody to rLipL32 protein with high titre. Results on western blotting and ELISA showed anti-LipL32 antibody was highly binding to rLipL32 protein. In contrast, MAT results for LipL32 antibody was found to be negative response, indicating that titre antibody to LipL32 does not positively correlate to MAT.en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1460
dc.identifier10.14334/Proc.Intsem.LPVT-2016-p.147-152
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/4918
dc.languageeng
dc.publisherIndonesian Center for Animal Science Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1460/1345
dc.rightsCopyright (c) 2017 Proceedings International Seminar on Livestock Production and Veterinary Technologyen-US
dc.sourceProceedings of International Seminar on Livestock Production and Veterinary Technology; 2016; 147-152en-US
dc.subjectLeptospira; Antibody Response; LipL32; MATen-US
dc.titlePreliminary Study: Characterisation of Antibody for rLipl32 Protein of Leptospiraen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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