Polymorphisms of Bovine Lymphocyte Antigen (BoLA DRB 3.2 Locus PstI) Gene in Bali Cattle Population from South Sulawesi Province
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Indonesian Center for Animal Science Research and Development (ICARD)
Abstract
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Immune Response in cattle is determined by polymorphism of major histocompatibility complex (MHC) gene mainly Bovine Lymphocyte Antigen. The aim of this study is to identify the genetic polymorphism of Bovine Lymphocyte Antigen (BoLA DRB.3) gene exon 2 as a marker for infectious disease resistance of Bali cattle in South Sulawesi, Indonesia. A total of 200 blood samples were extracted for DNA templated. It was amplified by using a PCR-RFLP method and digested with Pst1 restriction enzyme. Restriction of PCR product by Pst1 enzyme represented two digested fragments. The pp homozygous genotype showed two fragment 270 and 15 bp (not demonstrated). Pp heterozygous genotype showed four fragment 270, 226, 44 (not demonstrated), and 15 bp (not demonstrated). Reffering from this result this research, the frequency of allele P (0.925), allele p (0.075). The frequency genotype of PP (0.850), frequency of genotype Pp (0.150), polymorphism information content (0.129). Heterozygosity observed (0.150) and heterosygosity expected (0.139).
Keywords
Bali Cattle; MHC BoLA Gene; PCR-RLFP