Polymorphisms of Bovine Lymphocyte Antigen (BoLA DRB 3.2 Locus PstI) Gene in Bali Cattle Population from South Sulawesi Province

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dc.contributoren-US
dc.creatorIshak, Andi Baso L; South Sulawesi Assesment Institute of Agriculture Technology
dc.creatorDagong, MIA
dc.creatorNurul, P
dc.date2017-03-01
dc.date.accessioned2018-06-04T07:56:27Z
dc.date.available2018-06-04T07:56:27Z
dc.descriptionImmune Response in cattle is determined by polymorphism of major histocompatibility complex (MHC) gene mainly Bovine Lymphocyte Antigen. The aim of this study is to identify the genetic polymorphism of Bovine Lymphocyte Antigen (BoLA DRB.3) gene exon 2 as a marker for infectious disease resistance of Bali cattle in South Sulawesi, Indonesia. A total of 200 blood samples were extracted for DNA templated. It was amplified by using a PCR-RFLP method and digested with Pst1 restriction enzyme. Restriction of PCR product by Pst1 enzyme represented two digested fragments. The pp homozygous genotype showed two fragment 270 and 15 bp (not demonstrated). Pp heterozygous genotype showed four fragment 270, 226, 44 (not demonstrated), and 15 bp (not demonstrated). Reffering from this result this research, the frequency of allele P (0.925), allele p (0.075). The frequency genotype of PP (0.850), frequency of genotype Pp (0.150), polymorphism information content (0.129). Heterozygosity observed (0.150) and heterosygosity expected (0.139).en-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1424
dc.identifier10.14334/Proc.Intsem.LPVT-2016-p.19-23
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/4869
dc.languageeng
dc.publisherIndonesian Center for Animal Science Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1424/1325
dc.rightsCopyright (c) 2017 Proceeding International Seminar on Livestock Production and Veterinary Technologyen-US
dc.sourceProceedings of International Seminar on Livestock Production and Veterinary Technology; 2016; 19-23en-US
dc.subjectBali Cattle; MHC BoLA Gene; PCR-RLFPen-US
dc.titlePolymorphisms of Bovine Lymphocyte Antigen (BoLA DRB 3.2 Locus PstI) Gene in Bali Cattle Population from South Sulawesi Provinceen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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