Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P

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dc.contributor en-US
dc.creator Hadiarto, Toto; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creator Ma'sumah, Ma'sumah; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creator Riyanti, Eny I.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date 2016-08-15
dc.date.accessioned 2018-05-02T06:14:52Z
dc.date.available 2018-05-02T06:14:52Z
dc.date.issued 2016-08-15
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3984
dc.identifier 10.21082/jbio.v8n2.2012.p62-68
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/444
dc.description Azospirillum sp. which has the ability for nitrogenfixation and phosphate solubilization may support modernfarming in Indonesia that is mostly dependent on the usageof chemical fertilizer N, P, and K. Genetic quality ofAzospirillum was improved in this research to obtainsuperior characters toward phosphate solubilization so thatit can become more effective in use for farmers. To achievethis goal, Azospirillum was mutated by means ofelectroporation using transposon EZ-Tn5<kan-2>Tnp. Theelectrotransformation resulted in 20 out of 22 transformantstested contained the marker gen (npt). 10, 6 and 4 mutantshave increased, decreased and lost phosphate-solubilizingfunction, respectively. Mutant with elevated phosphatesolubilizingability may be selected further to be utilized asbiofertilizer while others may be useful for identification ofgenes responsible for phosphate solubilization. en-US
dc.format application/pdf
dc.language eng
dc.publisher Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3984/3319
dc.rights Copyright (c) 2016 Jurnal AgroBiogen en-US
dc.source 2549-1547
dc.source 1907-1094
dc.source Jurnal AgroBiogen; Vol 8, No 2 (2012): Agustus; 62-68 en-US
dc.title Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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