Pembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan P

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dc.contributoren-US
dc.creatorHadiarto, Toto; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorMa'sumah, Ma'sumah; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorRiyanti, Eny I.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.date2016-08-15
dc.date.accessioned2018-05-02T06:14:52Z
dc.date.available2018-05-02T06:14:52Z
dc.date.issued2016-08-15
dc.descriptionAzospirillum sp. which has the ability for nitrogenfixation and phosphate solubilization may support modernfarming in Indonesia that is mostly dependent on the usageof chemical fertilizer N, P, and K. Genetic quality ofAzospirillum was improved in this research to obtainsuperior characters toward phosphate solubilization so thatit can become more effective in use for farmers. To achievethis goal, Azospirillum was mutated by means ofelectroporation using transposon EZ-Tn5<kan-2>Tnp. Theelectrotransformation resulted in 20 out of 22 transformantstested contained the marker gen (npt). 10, 6 and 4 mutantshave increased, decreased and lost phosphate-solubilizingfunction, respectively. Mutant with elevated phosphatesolubilizingability may be selected further to be utilized asbiofertilizer while others may be useful for identification ofgenes responsible for phosphate solubilization.en-US
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3984
dc.identifier10.21082/jbio.v8n2.2012.p62-68
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/444
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3984/3319
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.source2549-1547
dc.source1907-1094
dc.sourceJurnal AgroBiogen; Vol 8, No 2 (2012): Agustus; 62-68en-US
dc.titlePembentukan Populasi Mutan Azospirillum dengan Menggunakan Transposon untuk Sifat Superior terhadap Pelarutan Pen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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