In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification

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dc.contributor en-US
dc.creator Budiarto, Kurniawan; Indonesian Ornamental Crops Research Institute (IOCRI), Jl. Raya Ciherang, Segunung Pacet Cianjur 43253, West Java, Indonesia Phone (62-263) 512607; Facs. (62-263) 514138
dc.creator Marwoto, Budi; Indonesian Ornamental Crops Research Institute (IOCRI), Jl. Raya Ciherang, Segunung Pacet Cianjur 43253, West Java, Indonesia Phone (62-263) 512607; Facs. (62-263) 514138
dc.creator Soehendi, Rudy; Indonesian Ornamental Crops Research Institute (IOCRI), Jl. Raya Ciherang, Segunung Pacet Cianjur 43253, West Java, Indonesia Phone (62-263) 512607; Facs. (62-263) 514138
dc.date 2016-10-06
dc.date.accessioned 2018-05-25T08:50:55Z
dc.date.available 2018-05-25T08:50:55Z
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/bpn/article/view/4244
dc.identifier 10.21082/blpn.v19n1.2013.p%p
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/2091
dc.description Modification of osmotic pressure of in vitro medium-term canbe applied to conserve carnation accessions. This successfulmethod inspired the use of nutrient modification technique tohave the same function as far as these concerned. The researchwas then, conducted to evaluate in vitro media for preservingseveral carnation accessions in low temperature conditions.The research was carried out at Indonesian Ornamental CropsResearch Institute (IOCRI) from January 2007 to March 2008.A randomized completely block design with 25 replicationswas used to accomplish the combination of two factors. Thefirst factor was six commercial carnation accessions, namelycv. Light Pink Candy, Aicardy, Torpedo, Malaga, President,and Rendezvouz. While the second factor dealt with fourformulations of conservation media i.e. ½ MS + DMSO 3% +4% sucrose, modified hyponex + 4% sucrose, modifiedhyponex (no sucrose) and ½ MS + 4% sucrose (control). Theresults showed that medium term in vitro conservation forcarnation were successfully conducted up to 10 months usingin ½ MS + DMSO 3% + 4% sucrose and modified hyponex +4% sucrose without any significant differences and decreasesin viability when transferred in to shoot induction media. In theabsent of sucrose, however, the plantlet survivals were only upto 8 months. No significant variation on viability and numberof survival plantlet was observed among the carnationaccessions in all media tested. en-US
dc.format application/pdf
dc.language eng
dc.publisher Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/bpn/article/view/4244/3583
dc.rights Copyright (c) 2016 Buletin Plasma Nutfah en-US
dc.source 2549-1393
dc.source 1410-4377
dc.source Buletin Plasma Nutfah; Vol 19, No 1 (2013): Juni en-US
dc.title In Vitro Medium-Term Conservation of Several Carnation Accessions through Nutrient Modification en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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