Phylogenetic analysis of HPAI H5N1 virus from duck swab specimens in Indonesia

Show simple item record Mutisari, Dewi Muflihanah Hendrawati, Ferra Putra, Hamdu Hamjaya Sulistyo, Kartika Priscillia 2021-10-13T06:15:02Z 2021-10-13T06:15:02Z 2021-06
dc.description.abstract Objective: A phylogenetic study was carried out on the avian influenza virus (AIV) isolated from a disease outbreak in Sidenreng Rappang Regency, South Sulawesi, Indonesia, in 2018. Material and Methods: Oropharyngeal swabs and organ samples were obtained from ducks that showed clinical symptoms: torticollis, fascial edema, neurological disorders, the corneas appear cloudy, and death occurs less than 1 day after symptoms appear. In this study, isolate A/duck/ Sidenreng Rappang/07180110-11/2018 from duck was sequenced and characterized. Results: It was found that each gene segment of the virus has the highest nucleotide homology to the Indonesian highly pathogenic avian influenza (HPAI) H5N1 clade Multiple alignments of the sample Hemagglutinin (HA) gene with the avian influenza references virus showed that the pattern of amino acid arrangement in the cleavage site PQRERRRK-RGLF is the characteristic of the HPAI virus. In addition, the HA gene contained Q222 (glutamine) and G224 (glycine), signifying a high affinity to avian receptor binding specificity (SA α2,3 Gal). Furthermore, there was no genetic reassortment of this virus based on the phylogenetic analysis of HA, NA, PB1, PB2, PA, NP, M, and NS genes. Conclusion: The HPAI H5N1 clade virus was identified in duck farms in South Sulawesi, Indonesia. en_US
dc.language.iso en en_US
dc.publisher Perpustakaan Balai Besar Veteriner Maros en_US
dc.subject Phylogenetic en_US
dc.subject HPAI en_US
dc.subject H5N1 en_US
dc.subject duck en_US
dc.subject swab en_US
dc.subject Research Subject Categories::L Animal production/Produksi Hewan::L73 Animal diseases/Penyakit Hewan en_US
dc.title Phylogenetic analysis of HPAI H5N1 virus from duck swab specimens in Indonesia en_US
dc.type Book en_US

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