Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR

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dc.contributor en-US
dc.creator Ladja, Fausiah T.; Loka Penelitian Penyakit Tungro Jl. Bulo Lanrang Rappang Sidrap, Sulawesi Selatan, Indonesia
dc.creator Hidayat, Sri Hendrastuti; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.creator Damayanti, Tri Asmira; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.creator Rauf, Aunu; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.date 2016-04-30
dc.date.accessioned 2018-05-07T04:05:42Z
dc.date.available 2018-05-07T04:05:42Z
dc.date.issued 2016-04-30
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/jpptp/article/view/3497
dc.identifier 10.21082/jpptp.v35n1.2016.p39-44
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/1302
dc.description Virus tungro disease is a serious problem to rice crop in a certain area of rice production in Indonesia. The disease is caused by a combined infection of Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV). Both viruses were reported to infect ratoon rice plants, weeds, and wild rice. The study was conducted to detect RTBV and RTSV on some weeds. Weed samples were collected from rice fields in West Java, Bali, West Nusa Tenggara, Papua, and West Sumatera. The detection of RTBV and RTSV were carried out using Polymerase Chain Reaction (PCR) and Reverse Transcription (RT) – PCR, employing coat protein gene specific primers. RTBV specific DNA fragment of ~1400 bp size was successfully amplified from various weed species including: F. miliacea, C. iria, M. vaginalis, L. adscendens, S. zeylanica, D. sanguinalis, and E. crusgalli. RTSV specific DNA fragment of ~787 bp size was successfully amplified from weed species of F. miliacea, L. octovalvis, and D. sanguinalis. RTBV or RTSV specific DNA fragment was not amplified from L. flava and P. distichum. Weed samples infected by both viruses did not show any tungro symptom. Virus detection based on molecular technique was able to determine the status of weed whether it is as an alternate host of viruses. Weeds sanitation prior to rice planting, therefore, should be considered as an integral part of virus disease management. en-US
dc.format application/pdf
dc.language eng
dc.publisher Pusat Penelitian dan Pengembangan Tanaman Pangan en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jpptp/article/view/3497/2959
dc.rights Copyright (c) 2016 Jurnal Penelitian Pertanian Tanaman Pangan en-US
dc.rights http://creativecommons.org/licenses/by-nc-sa/4.0 en-US
dc.source 2541-5174
dc.source 2541-5166
dc.source Jurnal Penelitian Pertanian Tanaman Pangan; Vol 35, No 1 (2016): April 2016; 39-44 en-US
dc.title Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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