Deteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCR

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dc.contributoren-US
dc.creatorLadja, Fausiah T.; Loka Penelitian Penyakit Tungro Jl. Bulo Lanrang Rappang Sidrap, Sulawesi Selatan, Indonesia
dc.creatorHidayat, Sri Hendrastuti; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.creatorDamayanti, Tri Asmira; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.creatorRauf, Aunu; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor Jl. Dramaga, Bogor, Indonesia
dc.date2016-04-30
dc.date.accessioned2018-05-07T04:05:42Z
dc.date.available2018-05-07T04:05:42Z
dc.date.issued2016-04-30
dc.descriptionVirus tungro disease is a serious problem to rice crop in a certain area of rice production in Indonesia. The disease is caused by a combined infection of Rice Tungro Bacilliform Virus (RTBV) and Rice Tungro Spherical Virus (RTSV). Both viruses were reported to infect ratoon rice plants, weeds, and wild rice. The study was conducted to detect RTBV and RTSV on some weeds. Weed samples were collected from rice fields in West Java, Bali, West Nusa Tenggara, Papua, and West Sumatera. The detection of RTBV and RTSV were carried out using Polymerase Chain Reaction (PCR) and Reverse Transcription (RT) – PCR, employing coat protein gene specific primers. RTBV specific DNA fragment of ~1400 bp size was successfully amplified from various weed species including: F. miliacea, C. iria, M. vaginalis, L. adscendens, S. zeylanica, D. sanguinalis, and E. crusgalli. RTSV specific DNA fragment of ~787 bp size was successfully amplified from weed species of F. miliacea, L. octovalvis, and D. sanguinalis. RTBV or RTSV specific DNA fragment was not amplified from L. flava and P. distichum. Weed samples infected by both viruses did not show any tungro symptom. Virus detection based on molecular technique was able to determine the status of weed whether it is as an alternate host of viruses. Weeds sanitation prior to rice planting, therefore, should be considered as an integral part of virus disease management.en-US
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/jpptp/article/view/3497
dc.identifier10.21082/jpptp.v35n1.2016.p39-44
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/1302
dc.languageeng
dc.publisherPusat Penelitian dan Pengembangan Tanaman Panganen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/jpptp/article/view/3497/2959
dc.rightsCopyright (c) 2016 Jurnal Penelitian Pertanian Tanaman Panganen-US
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.source2541-5174
dc.source2541-5166
dc.sourceJurnal Penelitian Pertanian Tanaman Pangan; Vol 35, No 1 (2016): April 2016; 39-44en-US
dc.titleDeteksi Virus Tungro pada Gulma Padi Sawah Menggunakan Teknik PCRen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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