Primordial germ cells profiles incubated for 24 hours in phosphate buffer saline [-] solution

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dc.contributoren-US
dc.creatorKostaman, Tatan; IRIAP
dc.creatorSopiyana, Soni; IRIAP
dc.date2018-03-02
dc.date.accessioned2018-06-04T06:48:04Z
dc.date.available2018-06-04T06:48:04Z
dc.date.issued2018-03-02
dc.descriptionGonadal development is a sequential process that can be divided into three major events: the PGCs migration, sex determination and gonadal differentiation. This study was aimed to see the development of PGCs isolated from the gonads of embryos after being incubated for 7 days and then was incubated using a solution of Phosphate Buffer Saline (PBS) [-]. The developing gonad can be isolated from 7 days old chick and can be incubated at a temperature of 37.8oC in a solution of PBS [-]: without Ca2+ and Mg2+. The release of gonadal PGC was observed within 1, 8, 16, and 24 hours after the embryonic gonad was placed in a PBS solution [-]. The results showed that PGCs can be separated from gonadal tissues and can be collected by entering the developing gonad to the PBS [-] solution. The highest percentage of PGCs and survival rate was obtained after gonad was incubated for 1 hour and was not different with 8 hours (P>0.05). Those result was significantly different (P<0.05) with the 16 and 24 hours incubation. The highest purity rate percentage was in the 8 hours incubation, but did not show a significant difference (P>0.05) with the 1 and 16 hours incubation. The percentage of the purity differed (P<0.05) after the 24 hours incubation. It can be concluded that the most appropriate incubation time to obtain PGCs from the KUB chicken embryonic gonad is no more than 8 hours.en-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1802
dc.identifier10.14334/jitv.v22i3.1802
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3168
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1802/1567
dc.rightsCopyright (c) 2018 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 22, No 3 (2017): SEPTEMBER 2017; 144-150en-US
dc.titlePrimordial germ cells profiles incubated for 24 hours in phosphate buffer saline [-] solutionen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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