Keragaman Genetik Pamelo Indonesia berdasarkan Primer Random Amplified Polymorphic DNA
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Date
2007-03-31
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Indonesian Center for Horticulture Research and Development
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ABSTRAK. Analisis keragaman genetik diperlukan untuk mengetahui hubungan kekerabatan 18 varietas jeruk pamelo Indonesia. Penelitian bertujuan mengetahui keragaman genetik beberapa varietas pamelo berdasarkan primer RAPD. Daun dari tunas muda berumur 20-25 hari diekstrak untuk mendapatkan bulk DNA. Setiap sampel DNA dari setiap varietas diamplifikasi menggunakan 15 primer RAPD dan diseparasi menurut metode elektroforesis. Hasil visualisasi fragmen pita DNA dihitung berdasarkan ada dan tidaknya pita DNA. Hasil penelitian menunjukkan bahwa 2 primer RAPD, yaitu OPN14 dan OPN16 membedakan varietas pamelo yang dianalisis. Tiga kelompok besar pamelo telah terkelompok dan menunjukkan kekerabatan yang dekat serta memperlihatkan kesamaan yang tinggi menurut daerah asal dan karakter buah.ABSTRACT. Agisimanto, D. and A. Supriyanto. 2007. Genetic Diversity of Pummelo Based on Primer Random Amplified Polymorphic DNA. Genetic variablitiy is needed to understand relationship among 18 pummelo varieties in Indonesia. The objective of the research was to characterize genetic diversity of some pummelo varieties in Indonesia based on RAPD primer. Leaves from young flush, 20-25 days, were extracted and amplified by 15 RAPD primers. Bands of DNA were scored based on their presence and absence. Two primers of OPN 14 and OPN 16 were selected to visualize band pattern of pummelo varieties. At least, 3 groups of pummelos were clustered that showed closely relationship and highly similarity by place of origin and fruit characteristics.