Bacterial expression of larval peritrophins of Chryosomya bezziana

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dc.creatorWijffels, Gene
dc.creatorVoucoloco, Tony
dc.creatorMuharsini, Sri
dc.creatorSupriyanti, Florentina
dc.date2014-02-17
dc.date.accessioned2018-06-04T06:46:50Z
dc.date.available2018-06-04T06:46:50Z
dc.date.issued2014-02-17
dc.descriptionThree candidate antigens, Chrysomya bezziana peritrophin-48, Chrysomya bezziana peritrophin-15 and Chrysomya bezziana peritrophin-42, were prepared for recombinant protein production in Escherichia coli using a variety of expression vectors. Cb peritrophin-48 was expressed as a recombinant protein possessing a carboxy-terminal hexaHis tag. Cb peritrophin-15 was expressed as both a glutathione S-transferase fusion protein and as an amino-terminal hexaHis tagged protein. The glutathione Stransferase Cb peritrophin-15 construct produced a heterogeneous group of fusion proteins. Cb peritrophin-42 was also expressed as an amino-terminal hexaHis tagged protein. The two putative domains of Cb peritrophin-42 were also separately expressed, again with amino-terminal hexaHis tags. Cultures of the hexaHis constructs Cb peritrophin-48, -15 and –42 were demonstrated to be useful for the production and purification of these protein antigens and were scaled-up for vaccine trials and protein characterization studies.   Key words: Chrysomya, peritrophic membrane, peritrophin, recombinant protein, hexaHis tag, GSTen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/196
dc.identifier10.14334/jitv.v5i3.196
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2630
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/196/196
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 5, No 3 (2000): SEPTEMBER 2000; p.170-176en-US
dc.titleBacterial expression of larval peritrophins of Chryosomya bezzianaen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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