Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical
| dc.contributor | en-US | |
| dc.creator | Winarto, Budi | |
| dc.date | 2011-12-02 | |
| dc.date.accessioned | 2019-10-09T09:43:39Z | |
| dc.date.available | 2019-10-09T09:43:39Z | |
| dc.description | Kultur anter merupakan salah satu teknologi haploid penting dalam produksi tanaman haploid ganda dan berhasil diaplikasikan pada berbagai jenis tanaman, namun aplikasi pada Anthurium belum pernah dilaporkan. Penelitian dan pengembangan kultur anter Anthurium yang difokuskan untuk mempelajari pengaruh glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus dilakukan di Laboratorium Kultur Jaringan Balai Penelitian Tanaman Hias dari bulan Januari sampai dengan September 2008. Tujuan penelitian ialah mengetahui pengaruh kombinasi konsentrasi glutamin dan serin terhadap induksi, pertumbuhan, dan regenerasi kalus pada kultur anter Anthurium. Spadik Anthurium andraeanum cv. Tropical, kalus hasil kultur anter serta medium Winarto dan Teixeira digunakan dalam studi ini. Glutamin dan serin pada konsentrasi 0, 250, 500, dan 750 mg/l diuji dalam percobaan ini. Percobaan disusun menggunakan rancangan acak lengkap pola faktorial dengan empat ulangan. Hasil studi menunjukkan bahwa penambahan glutamin dan serin pada medium terseleksi belum memberikan pengaruh yang signifikan terhadap induksi, pertumbuhan, dan regenerasi kalus. Glutamin pada konsentrasi 250 mg/l menginduksi potensi tumbuh anter hingga 48% dengan 21% anter beregenerasi dan 1,3 anter per perlakuan membentuk kalus. Sementara serin pada 500 mg/l merupakan konsentrasi yang paling potensial dalam induksi kalus dengan 55% potensi tumbuh anter, 24% anter beregenerasi, dan 1,4 anter per perlakuan membentuk kalus. Glutamin 250 mg/l merupakan konsentrasi terbaik dibanding konsentrasi yang lain dalam mendukung pertumbuhan dan regenerasi kalus. Perlakuan tersebut tanpa serin mampu menginduksi potensi pertumbuhan kalus hingga 77% dengan volume kalus mencapai 237 mm3 dan empat tunas dihasilkan per eksplan. Sementara perlakuan serin justru mereduksi pertumbuhan dan regenerasi kalus dan menstimulasi senesensi kalus yang berdampak pada pencoklatan dan kematiannya. Dari hasil penelitian ini dapat disarankan penggunaan glutamin dibanding serin dalam meningkatkan keberhasilan kultur anter Anthurium.Anther culture is one of important haploid technologies in producing double haploid lines and successfully applied in many plants, while the application in Anthurium is not reported yet. Research and development in anther culture of Anthurium focusing on studying the effect of glutamine and serine on callus induction, growth, and its regeneration was conducted at Tissue Culture Laboratory of Indonesian Ornamental Crops Research Institute from January untill September 2008. Objective of this study was to know the effect of glutamine and serine on callus induction, growth, and its regeneration in anther culture of Anthurium. Spadix of Anthurium andraeanum cv. Tropical, callus derived from anther and Winarto and Teixeira medium were utilized in the study. Glutamine and serine of 0, 250, 500, and 750 mg/l were tested in the experiments. Factorial experiment was arranged by completely randomized design with four replications. Results of the study indicate that addition of glutamine and serine in selected culture medium gave moderate significant effect on induction, growth, and regeneration of callus. Glutamine in 250 mg/l induced potential growth of anther up to 48% with 21% regenerated anthers and 1.3 anthers per treatment producing calli, while 500 mg/l of serine was better concentration in callus formation with 55% potential growth of callus, 24% regenerated anthers and 1.4 anthers per treatment producing calli. In growth and regeneration of callus, supplementation of serine reduced callus capacity in growth and production of shoots and stimulated callus senescence causing browning and death of it, while 250 mg/l glutamine exhibited positive effect on them. The treatment without serine was able to induce potential growth of callus up to 77% with 237 mm3 per callus and four shoots produced per explants. Results of the study suggest application of glutamine rather than serine in improving anther culture of Anthurium. | en-US |
| dc.format | application/pdf | |
| dc.identifier | http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/882 | |
| dc.identifier | 10.21082/jhort.v21n4.2011.p293-305 | |
| dc.identifier.uri | http://124.81.126.59/handle/123456789/7986 | |
| dc.language | eng | |
| dc.publisher | Indonesian Center for Horticulture Research and Development | en-US |
| dc.relation | http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/882/724 | |
| dc.rights | Copyright (c) 2013 Indonesian Center for Horticulture Research and Development | en-US |
| dc.rights | http://creativecommons.org/licenses/by-sa/4.0 | en-US |
| dc.source | Jurnal Hortikultura; Vol 21, No 4 (2011): DESEMBER 2011; 293-305 | en-US |
| dc.source | 2502-5120 | |
| dc.source | 0853-7097 | |
| dc.subject | Anthurium andraeanum cv. Tropical; Anther culture; Glutamine; Serine; Callus; Regeneration. | en-US |
| dc.title | Pengaruh Glutamin dan Serin terhadap Kultur Anter Anthurium andraeanum cv. Tropical | en-US |
| dc.type | info:eu-repo/semantics/article | |
| dc.type | info:eu-repo/semantics/publishedVersion | |
| dc.type | Peer-reviewed Article | en-US |