Genotype Spesific on Somatic Embryo and Germination of Soybean
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Date
2012-12
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IAARD Press
Abstract
This research aims to study somatic embryo development of four soybean genotypes and their germination and conversion into intact plant. The explants used were clumps of embryogenic callus containing globular stage embryo(s). This experiment was designed using entirely randomized factorial model. Four soybean genotypes were combined with nine origin induction medium and four combinations of histodifferentiation regulators medium. Result showed that genotype and origin induction medium interacted in promoting cotyledon-stage embryos. Interaction of Ceneng and IK-12 medium (NAA 53.71 μM + 2.4-D 67.87 μM) resulted the highest efficiency in inducing cotyledon-stage embryo. The highest average number of cotyledon-stage embryo was reached by
combination of 2.69 μM NAA + 13.32 μM BA. Genotype Ceneng indicated the highest percent of germination and index of vigour. CG76-10 has the fastest germination rate, however the conversion efficiency was poor in all treatments.
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Keywords
Embryogenic callused, cotyedone-stage embryo,vigour index.