The viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extender

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dc.creator., Yudi
dc.creatorArifiantini, I
dc.creatorPurwantara, B
dc.creatorYusuf, T.L
dc.date2013-06-21
dc.date.accessioned2018-06-04T06:47:42Z
dc.date.available2018-06-04T06:47:42Z
dc.date.issued2013-06-21
dc.descriptionThe objective of the experiment was to study the motility and viability of spermatozoa of fresh semen, and the quality of extended semen with different sperm concentration in Dimitropoulos-modified extender. Semen was collected using artificial vagina from three 4-8 year old stallions (different breed). Semen characteristics and quality was evaluated macro- and microscopically. For longevity evaluation, semen was stored at room and chilled temperature, and was evaluated for motility and viability every 3 h. Prior extension, semen was centrifugated at 3000 rpm for 20 minutes. The condensed sperm was re-suspended in Dimitropoulos (DV) supplemented with 50 mM fructose with the concentration of 200, 100 and 50 x 106 spz/mL. All samples were stored at room and chilled temperature, and was evaluated for motility and viability every 3 h and 12 h for room and chilled temperature. Results of the experiments indicated that fresh semen characteristics was fairly good. For longevity evaluation, semen with motility of 48.33 and 10.42% was observed at 3 h and 12 h after the onset of storage. The extended-semen with 50 x 106 spz/mL showed significantly higher in term of motility and viability (P<.05) than that with 200 x 106 spz/mL, but for that of 100 x 106 spz/mL. It is recommended that sperm concentration should be 50 x 106 spz/ml for a long period storage with reasonable good quality. Key words: Stallion, Semen, Sperm Concentration, Dimitropoulosen-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/593
dc.identifier10.14334/jitv.v13i1.593
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2998
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/593/602
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 13, No 1 (2008): MARCH 2008; p.35-42en-US
dc.titleThe viability of fresh and extended semen of stallion with different sperm concentration in Dimitropoulos-modified extenderen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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