The preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresis

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dc.creatorGholib, Djaenudin
dc.creator., Subiyanto
dc.date2014-02-25
dc.date.accessioned2018-06-04T06:47:35Z
dc.date.available2018-06-04T06:47:35Z
dc.date.issued2014-02-25
dc.descriptionThe filtrate antigen ofDermatophilus congolensis was prepared based on the Makinde method, whereas the whole cell antigen was based on the Bida and Kelley method. Filtrate antigen of Dermatophilus congolensis has been tested with positive serum from experimental animals, guinea pigs and sheep by means of immurrodiffitsion test and electrophoresis . Positive serum was produced by inoculation of whole cell antigen of D. congolensis to the animals . The results showed that the immunodiffusion test resulted in one and two precipitation lines with positive serum of sheep and guinea pigs respectively. Electrophoresis SDS-PAGE presented about 8 bands with molecular weight in the range from above 30 kD to more than 94 kD. The bands were then transferred into nitrocellulose membrane and gave positive reaction with positive serum from sheep.   Key words : Antigen, Dermatophilus congolensis, immunodiffusion, electrophoresisen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/117
dc.identifier10.14334/jitv.v3i3.117
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2956
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/117/117
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 3, No 3 (1998); p.197-201en-US
dc.titleThe preparation of Dermatophilus congolensis antigen and its testing by means of immunodiffusion test and electrophoresisen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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