EFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLI

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dc.contributoren-US
dc.creatorRiyadi, Imron; Indonesian Biotechnology Research Institute for Estate Crops Jalan Taman Kencana No.1, Bogor 16151, Indonesia
dc.creatorSumaryono, Sumaryono; Indonesian Biotechnology Research Institute for Estate Crops Jalan Taman Kencana No.1, Bogor 16151, Indonesia
dc.date2017-01-30
dc.date.accessioned2020-11-06T02:30:37Z
dc.date.available2020-11-06T02:30:37Z
dc.descriptionThe application of gamma irradiation on plant materials may increase the genetic variation of the offspring with useful traits. The experiment was conducted to determine the effect of irradiation dosage of gamma ray on growth and development of sago palm (Metroxylon sagu) calli. Friable calli of sago palm derived from suspension culture were used as a material source. The primary calli were initiated from apical meristematic tissues of sago palm suckers of Alitir variety from Merauke, Papua. The treatments used were dosage of gamma ray irradiation at 0, 5, 10, 15, 20 and 25 Gy. The treated calli were then subcultured on modified Murashige and Skoog (MMS) solid medium containing 3% sucrose and 0.1% activated charcoal and added with 1 mg l-1 2,4-D and 0.1 mg l-1 kinetin. The results showed that at all irradiation dosages, calli biomass increased significantly. The highest proliferation of calli biomass of 5.33 folds from the initial culture after 4 weeks was achieved at gamma irradiation of 25 Gy, whereas the lowest proliferation of calli biomass of 3.4 folds was achieved at control. The best development of embryogenic calli was obtained at 10 Gy that produced 100% somatic embryos, whereas the lowest somatic embryo formation at 0% was obtained at 0 and 25 Gy after one subculture. High response of somatic embryo induction to gamma irradiation at 10 Gy may increase production of somatic embryos. These results can be used in in vitro breeding of sago palm via mutagenesis to create new elite varieties.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/3622
dc.identifier10.21082/ijas.v17n1.2016.p35-40
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/10493
dc.languageeng
dc.publisherIndonesian Agency for Agricultural Research and Developmenten-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/3622/5228
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3622/571
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3622/572
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3622/573
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3622/574
dc.rightsCopyright (c) 2017 Indonesian Journal of Agricultural Scienceen-US
dc.rightshttp://creativecommons.org/licenses/by-sa/4.0en-US
dc.sourceIndonesian Journal of Agricultural Science; Vol 17, No 1 (2016): April 2016; 35-40en-US
dc.source2354-8509
dc.source1411-982X
dc.subjectMetroxylon sagu; gamma irradiation; embryogenic calli; somatic embryoen-US
dc.titleEFFECT OF GAMMA IRRADIATION ON THE GROWTH AND DEVELOPMENT OF SAGO PALM (Metroxylon sagu Rottb.) CALLIen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
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