Molecular characterization of infectious laryngotracheitis virus (ILTV) isolates from outbreak cases at Lipa City, Batangas Province, The Philippines

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dc.creatorSaepulloh, Muharam
dc.date2014-02-21
dc.date.accessioned2018-06-04T06:48:16Z
dc.date.available2018-06-04T06:48:16Z
dc.date.issued2014-02-21
dc.descriptionInvestigations were carried out to identify molecular character of infectious laryngotracheitis virus (ILTV) isolates from commercial layer chicken farm located at Lipa City, Batangas Province, the Philippines using western blotting. The virus was first isolated in chorio allantoic membrane (CAM). A-total of five isolates (#IV, #VI-C28, #VI-C29, #VI-C30, and #VII) produced typical plaque lesions in CAM at second passages such as yellowish plaques with opaque edges. Furthermore, five isolates were then characterized by western blotting on 7.5% of acrylamide. These results showed that Chicken antisera to the ILTV strain NS-175 (as standard sera), and rabbit antisera to vaccine strain (BAL-ILT) recognized four major viral protein with molecular weight of 205, 160, 85 and 60 kDa of isolates #VI-C28, #VI-C29, #VI-30 and #VII. While the isolate # IV produced viral protein of 205 and 85 kDa. The same four viral proteins were recognized by both ILTV antisera, indicating that the viral proteins of the vaccine strain and ILTV local isolates from Lipa City, Batangas Province, the Philippine had cross-reactivity. Thus, this cross reactivity may cause the effective protection afforded by the vaccine strain in the field.   Key words: Infectious laryngotracheitis virus, CAM, western blotting, chickenen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/425
dc.identifier10.14334/jitv.v9i1.425
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3252
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/425/434
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 9, No 1 (2004): MARCH 2004; p.26-36en-US
dc.titleMolecular characterization of infectious laryngotracheitis virus (ILTV) isolates from outbreak cases at Lipa City, Batangas Province, The Philippinesen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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