Antioxidant Activities of GMB 7 Variety of Tea at Different Altitude

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dc.contributorPT. Tambi Wonosoboen-US
dc.contributorPT. Tambi Wonosoboid-ID
dc.creatorMartono, Budi; Balai Penelitian Tanaman Industri dan Penyegar
dc.creatorFalah, Syamsul; Institut Pertanian Bogor
dc.creatorNurlaela, Eneng; Institut Pertanian Bogor
dc.date2016-03-31
dc.date.accessioned2018-06-04T06:39:15Z
dc.date.available2018-06-04T06:39:15Z
dc.date.issued2016-03-31
dc.descriptionGMB 7 is a tea variety which has high productivity and a potential source of natural antioxidants, grow well at low, medium, and high altitude. The research aimed to analyze the effect of altitude on antioxidant activity, total phenolic and total flavonoids content of GMB 7 tea variety. The research was conducted at the Biopharmacy Laboratory, Bogor Agricultural University, from November 2014 to February 2015. The treatments tested were of tea shoots extracts taken from three different altitudes at PT Tambi tea plantations,Wonosobo, at 690, 1.280, and 1.890 m above sea level. The tea shoots (100 g)  were mixed in aquades with the ratio of 1:10, extracted with hot water (90°C; 30 minutes). The extracts were then filtered and evaporated using rotary vacuum evaporator (50 rpm; 60°C) to obtain a raw dried extract. Total phenolic was tested with Follin-Ciocalteu method at a wavelength of 765 nm while the total flavonoids was tested using AlCl3 method at a wavelength of 510 nm. The antioxidant activity was determined by diphenylpicrylhydrazyl method (DPPH) at a wavelength of 517 nm.  The results showed that total phenolics, total flavonoids, and IC50 at an altitude of 690 m asl  is higher compared to those at  1.280, and 1.890 m above sea level , respectively by 19%; 290.62 GAE mg/g extract, 125  CE mg/g extract,  and 5.48 ppm. The total phenolic and flavonoid content significantly correlated with the antioxidant activity (IC50).en-US
dc.descriptionGMB 7 merupakan varietas unggul teh yang mempunyai produktivitas tinggi dan berpotensi sebagai sumber antioksidan alami, dapat tumbuh dengan baik di dataran rendah, sedang, dan tinggi. Tujuan penelitian adalah menganalisis pengaruh ketinggian tempat terhadap aktivitas antioksidan, kandungan fenolik, dan flavonoid pada teh varietas GMB 7. Penelitian dilakukan di Laboratorium Biofarmaka Institut Pertanian Bogor, mulai November 2014 sampai Februari 2015. Perlakuan yang diuji adalah ekstrak pucuk teh yang diambil dari tiga ketinggian tempat di perkebunan teh PT Tambi, Wonosobo, yaitu 690, 1280, dan 1890 m dpl. Serbuk pucuk teh sebanyak 100 g ditambahkan akuades dengan perbandingan 1:10, diekstraksi dengan air panas (90°C; 30 menit). Ekstrak disaring dan filtratnya dikeringkan menggunakan rotary vacuum evaporator (50 rpm; 60°C) sampai diperoleh ekstrak kasar kering. Fenolik total diuji dengan metode Follin-Ciocalteu pada panjang gelombang 765 nm, sedangkan flavonoid total diuji menggunakan metode AlCl3 pada panjang gelombang 510 nm. Aktivitas antioksidan ditentukan dengan metode diphenylpicrylhydrazyl (DPPH) pada panjang gelombang 517 nm. Hasil penelitian menunjukkan nilai fenolik dan flavonoid total, serta nilai IC50 GMB 7 pada ketinggian 690 m dpl lebih tinggi dibandingkan pada ketinggian 1.280 dan 1.890 m dpl,  masing-masing sebesar 290,62 GAE mg/g ekstrak, 125 CE mg/gekstrak, dan 5,48 ppm. Kandungan fenolik dan flavonoid total berkorelasi sangat nyata dengan aktivitas antioksidan (IC50).  id-ID
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/bultri/article/view/6062
dc.identifier10.21082/jtidp.v3n1.2016.p53-60
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2545
dc.languageind
dc.publisherPusat Penelitian dan Pengembangan Perkebunanen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/bultri/article/view/6062/5247
dc.rightsCopyright (c) 2016 Jurnal Tanaman Industri dan Penyegaren-US
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.source2528-7222
dc.source2356-1297
dc.sourceJurnal Tanaman Industri dan Penyegar; Vol 3, No 1 (2016): Jurnal Tanaman Industri dan Penyegar; 53-60en-US
dc.sourceJurnal Tanaman Industri dan Penyegar; Vol 3, No 1 (2016): Jurnal Tanaman Industri dan Penyegar; 53-60id-ID
dc.titleAntioxidant Activities of GMB 7 Variety of Tea at Different Altitudeen-US
dc.titleAktivitas Antioksidan Teh Varietas GMB 7 Pada Beberapa Ketinggian Tempatid-ID
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
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