Effect of different diluents on the quality of sperm sexing cows with a gradient albumin (egg white)

Simple item page
dc.creatorM, Ervandi
dc.creatorT, Susilawati
dc.creatorS, Wahyuningsih
dc.date2014-01-07
dc.date.accessioned2018-06-04T06:48:22Z
dc.date.available2018-06-04T06:48:22Z
dc.date.issued2014-01-07
dc.descriptionDamage of spermatozoa membrane in sexing procces with albumin gradient can decrease the quality of spermatozoa, thus to solve that need diluents for protect spermatozoa membrane to make good quality. The aim of this research is to know the best diluents between Andromed and CEP2 (cauda epididymal plasma) + 0% of yolk to keep the quality of Limousin Cow’s Spermatozoa from sexing procces with albumin’s gradient. Parameters measured for the quality of spermatozoa were : spermatozoa motility, spermatozoa viability, spermatozoa abnormality, spermatozoa concentration, and spermatozoa motility, membrane integrity, spermatozoa capacity, and acrosom reaction. Observation of membrane integrity using HOS (Hypoosmotic Swelling Test), observation of capacity and acrosom reaction using fluoresen CTC (Chortetracycline) dye. Result show that Andomed diluents and CEP2 + 10% yolk in top layer and bottom (X) and (Y) layer can keep the quality of Limousin Cow’s spermatozoa in sexing procces include spermatozoa motility (X 62,5%, Y 58%,) (X 56,5%, Y 55%), viability (X 91,64%, Y 91,51%) (X 89,87%, Y 93,51%, spermatozoa’s concentration (X 643 million /ml, Y518 million /ml) (X648 million/ml, Y517 million/ml), total spermatozoa are motil (X373,60 juta/ml, Y187,80 million/ml) (X 296,03 miliion/ml, Y 187,38 million/ml) , and have low abnormality (X 4,11%, Y 4,38%), (X 6,42%, Y 4,04%). Andromed diluents and CEP2 + 10% yolk can keep the integrity of spermatozoa membrane (X 87,60%, Y 80,26%) (X 79,61%, Y 81,03%), remains well and have the spermatozoa are not yet capacitation(X 87,60%, Y83,71%) (X 79,61%, Y 81,03%) remains high, spermatozoa capacity (X 9,76%, Y 9,92%) (X 13,45%, Y 8,95%), dan acrosom reaction (X 4,11%, Y 4,38%), (X 6,42%, Y 4,04%) remains low. Andromed diluents was not different (P > 0,05) from CEP2 diluents + 10% yolk in keeping the quality of Limousin Cow Spermatozoa from the sexing process with albumin’s gradient (egg white). Key Words: Semen, Andromed, CEP+10% Yolk, Albumin’s Gradient, Sexingen-US
dc.formatapplication/pdf
dc.formattext/html
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/319
dc.identifier10.14334/jitv.v18i3.319
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3279
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/319/321
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/319/322
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 18, No 3 (2013): SEPTEMBER 2013; p.177-184en-US
dc.titleEffect of different diluents on the quality of sperm sexing cows with a gradient albumin (egg white)en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Effect of different diluents on the quality of sperm sexing cows with a gradient albumin (egg white).pdf
Size:
180.53 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Jurnal Ilmu Ternak dan Veteriner