The difference of serological responses between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine
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Date
2014-02-25
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Indonesian Animal Sciences Society
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The purposes of this research were to observe serological response differences of brucellosis between naturally infected, experimentally infected, and vaccinated cattle with Brucella abortus strain 19 vaccine . Identification of naturally infected cattle with B. abortus was carried out bacteriologically on milk samples collected from sero-positive brucellosis, while to determine serological responses blood samples were collected with 2 week interval from naturally and experimentally infected, and vaccinated cattle . Futhermore, the collected serum was examined serologically by using complement fixation test (CFT) and enzyme-linked immunosorbent assay (ELISA, indirect and competitive) . The result indicated that antibody titres (ELISA unit) of vaccinated cattle reached the peak at week -6 . This antibody titre was relatively lower and decreased quicker than naturally or experimentally infected cattle.