PRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS OF CACAO: THE EFFECT OF EXPLANT TYPES AND PLANT GROWTH REGULATORS

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dc.contributoren-US
dc.creatorAjijah, Nur; Indonesian Industry and Freshener Crops Research Institute
dc.creatorHartati, Rr Sri; Indonesian Center for Estate Crops Research and Development
dc.date2020-01-17
dc.date.accessioned2020-11-06T02:30:47Z
dc.date.available2020-11-06T02:30:47Z
dc.descriptionThe success of cacao somatic embryogenesis is affected by many factors, including the basal salt medium, the genotype, the explant type, and the concentration and composition of plant growth regulators (PGRs). The study aimed to evaluate the effects of PGRs composition on the primary somatic embryo (PSE) response and the effect of explant type and PGRs composition used in inducing PSE on the secondary somatic embryogenesis (SSE) response. PSEs were induced from basal petal and staminoid explants of MCC 01 and MCC 02 clones on DKW medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1 or 2,4-D 2 mg l-1 + kinetin 0.125 – 0.250 mg l-1 + thidiazuron (TDZ) 2.5 – 5 µg l-1 or 2,4-D 2 mg l-1 + TDZ 10 µg l-1. Genotype, explant type, and PGR composition dependently affected PSE response. The best PSE response was obtained from staminoid explant of MCC 02 clone on medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1 (20%, 9 embryos). The explant type and PGR composition used in inducing PSEs affect the SSE response. The highest  SSE response of MCC 01 clone was obtained from petal explant with medium containing 2,4-D 2 mg l-1 + kinetin 0.5 mg l-1. The formation of SSEs could increase the multiplication rate of MCC 01 clone by 7 times.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/11174
dc.identifier10.21082/ijas.v20n2.2019.p69-76
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/10511
dc.languageeng
dc.publisherIndonesian Agency for Agricultural Research and Developmenten-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/11174/8886
dc.rightsCopyright (c) 2020 Indonesian Journal of Agricultural Scienceen-US
dc.rightshttp://creativecommons.org/licenses/by-sa/4.0en-US
dc.sourceIndonesian Journal of Agricultural Science; Vol 20, No 2 (2019): December 2019; 69-76en-US
dc.source2354-8509
dc.source1411-982X
dc.subjectexplants; PGRs; primary somatic embryogenesis; secondary somatic embryogenesis; Theobroma cacao L.en-US
dc.titlePRIMARY AND SECONDARY SOMATIC EMBRYOGENESIS OF CACAO: THE EFFECT OF EXPLANT TYPES AND PLANT GROWTH REGULATORSen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typeen-US
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