ANALYSIS OF GENETIC DIVERSITY IN TWELVE CULTIVARS OF PEA BASED ON MORPHOLOGICAL AND SIMPLE SEQUENCE REPEAT MARKERS

Singh, Brijesh Kumar; Department of Agricultural Biotechnology 
Bidhan Chandra Krishi Viswavidyalaya
Mohanpur-741252, Nadia(W.B); Sutradhar, Monoj; Department of Agricultural Biotechnology  Bidhan Chandra Krishi Viswavidyalaya; Singh, Amit Kumar; Central Agriculture University, College of Horticulture and Forestry; Singh, Ajay Kumar; Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology; Vyas, Rajendra Prakash; Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology

ANALYSIS OF GENETIC DIVERSITY IN TWELVE CULTIVARS OF PEA BASED ON MORPHOLOGICAL AND SIMPLE SEQUENCE REPEAT MARKERS

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Authors

Singh, Brijesh Kumar; Department of Agricultural Biotechnology Bidhan Chandra Krishi Viswavidyalaya Mohanpur-741252, Nadia(W.B)

Sutradhar, Monoj; Department of Agricultural Biotechnology Bidhan Chandra Krishi Viswavidyalaya

Singh, Amit Kumar; Central Agriculture University, College of Horticulture and Forestry

Singh, Ajay Kumar; Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology

Vyas, Rajendra Prakash; Department of Genetics and Plant Breeding, Candra Shekar Azad University of Agriculture and Technology

Publisher

Indonesian Agency for Agricultural Research and Development

Description

Pea(Pisum sativum L.)is the second most important legume crop worldwide after chickpea (Cicer arietinum L.) and valuable resources for their genetic improvement. This study aimed to analyze genetic diversity of pea cultivars through morphological and molecular markers. The present investigation was carried out with 12 pea cultivars using 28 simple sequence repeat markers. A total of 60 polymorphic bands with an average of 2.31 bands per primer were obtained. The polymorphic information content, diversity index and resolving power were ranged from 0.50 to 0.33, 0.61 to 0.86 and 0.44 to 1.0 with an average of 0.46, 0.73 and 0.76, respectively. The 12 pea cultivars were grouped into 3 clusters obtained from cluster analysis with a Jaccardd’s similarity coefficient range of 0.47-0.78, indicating the sufficient genetic divergence among these cultivars of pea. The principal component analysis showed that first three principal components explained 86.97% of the total variation, suggesting the contribution of quantitative traits in genetic variability. The contribution of 32.59% for number of seeds per plant, stem circumference, number of pods per plant and number of seeds per pod in the PC1 leads to the conclusion that these traits contribute more to the total variation observed in the 12 pea cultivars and would make a good parental stock material. Overall, this SSR analysis complements morphological characters of initial selection of these pea germplasms for future breeding program.

Keywords

Genetic diversity; pea cultivars; pisum sativum; simple sequence repeat

URI

https://repository.pertanian.go.id/handle/123456789/13313

Collections

Indonesian Journal of Agricultural Science

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