diversity of myiasis fly, Chrysomya bezziana population in Indonesia based on mitochondrial DNA

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dc.creatorWardhana, April H
dc.creatorMuharsini, S
dc.creatorAsmara, Widya
dc.date2012-02-05
dc.date.accessioned2018-06-04T06:48:21Z
dc.date.available2018-06-04T06:48:21Z
dc.date.issued2012-02-05
dc.descriptionChrysomya bezziana is a fly causing myiasis in most livestock in Indonesia. To date, the genetic diversity of C. bezziana has been argued among researchers. The aim of this study was to analyse genetic diversity of C. bezziana population in Indonesia using mitochondrial cytochrome b gene (cyt b) as a marker. The preliminary study showed that the larvae instar III stadium (L3) was the most appropriate sample for molecular analysis and identification. Twenty-four L3 were collected from cattles, buffalos and horses in Bogor, Makassar and East Sumba. DNA samples were isolated from muscle tissue of the larvae. The fragment of cyt b gene (279 bp) was amplified using primer CB3FC-NINFA. The PCR product was subsequently purified, sequenced and analysed using PAUP version 4. The results showed that C. bezziana from Indonesia was different with those from Asia, basicaly for DNA sequences, however it had similar to those from Papua New Guinea. Two haplotypes were identified i.e. haplotype 6 (Makassar haplotype that similar to those from Papua New Guinea) and haplotype 7 (Bogor haplotype that similar to those from Makassar and East Sumba). Key words: Chrysomya bezziana, genetic diversity, mitochondrial DNAen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/416
dc.identifier10.14334/jitv.v9i2.416
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3275
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/416/425
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 9, No 2 (2004): JUNE 2004; p.108-114en-US
dc.titlediversity of myiasis fly, Chrysomya bezziana population in Indonesia based on mitochondrial DNAen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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