Efikasi Gen RB pada Tanaman Kentang Transgenik Katahdin SP904 dan SP951 terhadap Empat Isolat Phytophthora infestans dari Jawa Barat

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dc.contributoren-US
dc.creatorAmbarwati, Alberta Dinar; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorSumaraw, S M; Departemen Proteksi Tanaman, Fakultas Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16680 Telp. (0251) 8629364; Faks. (0251) 8629362
dc.creatorPurwito, Agus; Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16680 Telp. (0251) 8629352; Faks. (0251) 8629353
dc.creatorHerman, Muhammad; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
dc.creatorSuryaningsih, E.; Balai Penelitian Tanaman Sayuran, Jl. Tangkuban Perahu 517, Kotak Pos 8413, Lembang 40391 Telp. (022) 2786245; Faks. (022) 2786416, 2786025
dc.creatorAswidinnoor, Hajrial; Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16680 Telp. (0251) 8629352; Faks. (0251) 8629353
dc.date2011-04-01
dc.date.accessioned2018-05-02T06:15:03Z
dc.date.available2018-05-02T06:15:03Z
dc.date.issued2011-04-01
dc.descriptionEfficacy of RB gene in transgenic potato Katahdin SP904and SP951 to West Java isolates of Phytophthorainfestans. A. Dinar Ambarwati, S.M. Sumaraw, AgusPurwito, M. Herman, E. Suryaningsih, and HajrialAswidinnoor. Potato late blight, caused by Phytophthorainfestans is one of the most devastating plant disease. Potatoyield losses due to this disease ranged from 47-100%. Amajor late blight resistance gene, called RB, previously wasidentified in the wild potato species Solanumbulbocastanum. RB gene has been integrated into cultivatedpotato Katahdin using Agrobacterium-mediated transformation,and showed durable and broad spectrum resistanceeither in laboratory assay or in confined field trial. Evaluationof transgenic Katahdin SP904 and SP951 was conducted toverify whether the RB gene with broad spectrum to allknown races of P. infestans in the United States and inToluca, Mexico was also effective against P. infestansisolates in Indonesia. Efficacy of RB gene was evaluated forfoliar and tuber resistance to West Java isolates. TransgenicKatahdin were more resistant in foliar than non transgenicplants, at 14 days after inoculation. Diseases intensity oftransgenic Katahdin SP904 and SP951 were 19.8-43.8%,whereas non transgenic Katahdin, Granola, and Atlanticwere 46.9-100%. In contrast to the foliar resistancephenotype, RB-containing tubers in transgenic Katahdin didnot exhibit increased resistance to Lembang, Pangalenganand Galunggung isolates. Tubers of transgenic KatahdinSP904, SP951, and non transgenic Katahdin showed lesionvolume of 0.93, 0.91, and 0.91 cm3, respectively. RB gene intransgenic Katahdin showed efficacy against late blight P.infestans in foliar, but did not showed efficacy in tuber.Transgenic Katahdin RB thus providing a potential source ofresistance for breeding programs.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3766
dc.identifier10.21082/jbio.v7n1.2011.p28-36
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/499
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3766/3116
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.source2549-1547
dc.source1907-1094
dc.sourceJurnal AgroBiogen; Vol 7, No 1 (2011): April; 28-36en-US
dc.titleEfikasi Gen RB pada Tanaman Kentang Transgenik Katahdin SP904 dan SP951 terhadap Empat Isolat Phytophthora infestans dari Jawa Baraten-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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