Effect of maturation periods and leukaemia inhibitory factor on in vitro bovine embryo development

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dc.contributoren-US
dc.creatorMargawati, Endang Tri
dc.date2014-02-25
dc.date.accessioned2018-06-04T06:48:38Z
dc.date.available2018-06-04T06:48:38Z
dc.date.issued2014-02-25
dc.descriptionThe period of in vitro maturation,~20 vs 24 hours) with of without supplementation of :,~uk_ni;ia inhib toryy factor (LIF) (0, 500, 1000 or 2000 U/ml) was studied on bovine embryo development in vitro in a 2 x 4 factotial experiment w id oestgnoo i, a randomized block design . A total of 870 bovine oocytes were used . Besides embryo development, cell numbers of blastocysts v. : ;re also co , mted in order to study the quality of the embryos . Oocytes were matured in a modified TCM199 medium containing 10 ug/nil of FS-14 and LP., I 1~ t1L rstradiol, fertilized in TALP and cultured in SOF/AABSA medium. There was no interaction between maturation periods and LIF doses on embryo development (P>0 .05). Maturation periods, however, affected (P<0.05) blastocyst rates but did not for cleavage o, oocytes and the percentage of oocytes that developed into blastocysts . LIF doses during in vitro maturation did not affect embryo development (P>0 .05) . Cell numbers of blastocysts were also not affected by maturation periods and LIF doses (P<0 .05), however 20 h in vitro maturation and supplementation of LIF doses tended to increase the cell numbers. This study suggests that 20 h maturation increases blastocyst rates and that supplementation with LIF during maturation does not affect the quality of embryos produced in vitro.en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/26
dc.identifier10.14334/jitv.v1i3.26
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3410
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/26/26
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 1, No 3 (1995); p.149-154en-US
dc.titleEffect of maturation periods and leukaemia inhibitory factor on in vitro bovine embryo developmenten-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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