Effectivity of PCR and AGID methods to detect of enzootic bovine leukosis in Indonesia

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dc.contributoren-US
dc.creatorM, Saepulloh
dc.creatorI, Sendow
dc.date2015-03-12
dc.date.accessioned2018-06-04T06:48:35Z
dc.date.available2018-06-04T06:48:35Z
dc.date.issued2015-03-12
dc.descriptionEnzootic Bovine Leucosis (EBL) is one of viral diseases in cattle caused by bovine leukemia virus (BLV), from Retroviridae. The virus can be detected using severals methods such as Polymerase Chain Reaction (PCR), while antibody can be detected using Agar Gel Immunodifussion (AGID). The aim of this experiment was to study the effectivity of PCR and AGID methods to detect enzootic bovine leukosis virus in Indonesia. Samples of peripheral blood leukocyte (PBL) were collected from cattles those with and without showing clinical signs. A total of 307 blood  and serum samples were  tested against BLV using PCR and AGID tests, while 21 semen samples which were from similar animals for blood collection were collected only for PCR test. The results indicated that twelve cattles have positive  results with PCR test in PBL, but from those cattles  only seven were positive with AGID. On the other hand, the PCR did not detect EBL in 21 bovine semen samples tested, although one sample gave positive result with PCR in PBL. This results indicated that PCR method from blood samples was more sensitive than that AGID method. The PCR detection was also more sensitive for PBL than that for semen samples.en-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1120
dc.identifier10.14334/jitv.v20i1.1120
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3385
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1120/pdf
dc.rightsCopyright (c) 2015 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 20, No 1 (2015): MARCH 2015; p.71-78en-US
dc.titleEffectivity of PCR and AGID methods to detect of enzootic bovine leukosis in Indonesiaen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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