Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers

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dc.contributorBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanianen-US
dc.creatorRosalia, Dea; Jurusan Budidaya , Fakultas , Universitas Brawijaya, Jalan Veteran, Malang 65145 Indonesia
dc.creatorLestari, Puji; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
dc.creatorSoegianto, Andy; Jurusan Budidaya , Fakultas , Universitas Brawijaya, Jalan Veteran, Malang 65145 Indonesia
dc.creatorSaptadi, Darmawan; Jurusan Budidaya , Fakultas , Universitas Brawijaya, Jalan Veteran, Malang 65145 Indonesia
dc.creatorSutanto, Agus; Balai Penelitian Tanaman Buah, Solok Sumatera Barat
dc.creatorNugroho, Kristianto; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
dc.creatorTerryana, Rerenstradika T.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
dc.creatorTasma, I Made; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
dc.creatorRoostika, Ika; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
dc.date2020-07-17
dc.descriptionBanana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/10671
dc.identifier10.21082/jbio.v16n1.2020.p17-24
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/10671/9085
dc.rightsCopyright (c) 2020 Jurnal AgroBiogenen-US
dc.rightshttp://creativecommons.org/licenses/by-nc-sa/4.0en-US
dc.sourceJurnal AgroBiogen; Vol 16, No 1 (2020): June; 17-24en-US
dc.source2549-1547
dc.source1907-1094
dc.subjectBanana; cross; allele,;F1 populationen-US
dc.titleConfirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markersen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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