Vaccine Development Strategy for Myiasis Caused by the Larvae of Chrysomya Bezziana (The Old World Screwworm Fly)

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dc.creatorMuharsini, Sri
dc.date2005-06-01
dc.date.accessioned2018-06-04T07:49:29Z
dc.date.available2018-06-04T07:49:29Z
dc.descriptionIndonesia is an endemic area for myasis caused by the larvae of Chrysonrya bezziana . So far, the controlling method for myiasis is using insecticides such as organophosphate and coumaphos compounds . Sterile Insect Technique (SIT) was a successful method for myiasis control, however, this method is expensive, beside it needs a large amount of insecticide. Therefore, vaccinations as an alternate controlling method for myiasis which relatively cheap, environment friendly and in accordance with the sustainable agriculture concept are needed . The strategy of myiasis vaccine development has been done by the Indonesia Research Institute for Veterinary Science in collaboration with IUC-ITB, Bandung and CSIRO, Brisbane-Australia, are briefly discussed in this paper. The first step was to identify and purify the protective antigens from excretory/secretory material and peritrophic membrane. The excretory/secretory material was purified and analysed using amino-terminal sequencing and resulted trypsin and chymotrypsin at 26 kDa and 28 kDa, respectively . The peritrophic membrane was then fractionated and resulted candidate antigens of Cb 15, Cb42 and Cb48 . The three antigens were expressed into bacterial Eschericia coli and yeast Pichia pastoris resulting different yield depending on each protein character . The strategy of myiasis vaccine could be used as an alternate way for vaccine development for other parasite diseases in Indonesia. Key words: Chrysoniya bezziana, vaccine, excretory/secretory, peritrophic membraneen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/832
dc.identifier10.14334/wartazoa.v15i2.832
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/4740
dc.languageeng
dc.publisherIndonesian Center for Animal Research and Developmenten-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/wartazoa/article/view/832/841
dc.source2354-6832
dc.source0216-6461
dc.sourceWARTAZOA. Indonesian Bulletin of Animal and Veterinary Sciences; Vol 15, No 2 (2005): JUNE 2005; 102-110en-US
dc.titleVaccine Development Strategy for Myiasis Caused by the Larvae of Chrysomya Bezziana (The Old World Screwworm Fly)en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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