Application of Dried Blood Sample on Whatman Filter Paper for Detection of Trypanosoma evansi from Cattle in Central Kalimantan by Internal Trascriber Spacer-1 Polymerase Chain Reaction

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dc.contributoren-US
dc.creatorSawitri, Dyah Haryuningtyas; Indonesian Research Center for Veterinary Science
dc.creatorWardhana, April H; Indonesian Research Center for Veterinary Science
dc.creatorDewi, Dias A; Indonesian Research Center for Veterinary Science
dc.creatorEkawasti, Fitrine; Indonesian Research Center for Veterinary Science
dc.creatorWidjaja, E; Indonesian Center for Agricultural Technology Assessment and Development
dc.date2017-03-31
dc.date.accessioned2018-06-04T07:56:33Z
dc.date.available2018-06-04T07:56:33Z
dc.descriptionTrypanosoma evansi is a hemoflagella parasite that infects cattle as agents of Surra. The disease causes great economic losses due to decrease in production and death. Rapid and accurate method for early detection is necessary so that the appropriate control can be implemented. In recent years, PCR has been widely applied for detection of Trypanosoma with higher sensitivity. Trypanosoma-species-specific-DNA-sequences multi copy can be used as a target parasite identification. Internal transcribed spacer regions 1 (ITS-1) is a useful marker for descriminating trypanosomes species. The aims of this study was to detect T. evansi in cattle from Central Kalimantan using ITS-1 PCR. A total of 44 bovine blood samples were colleted using Whatman filter paper from Lamandau District of Central Kalimantan Province. PCR was carried out using ITS-1 primers on DNA templete that was isolated from dried blood. The results showed that 27 out of 44 samples (61.4%) were positive for T. evansi. Lamandau District was suspected to be endemic area of Surra and therefore requires a special attention.en-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1479
dc.identifier10.14334/Proc.Intsem.LPVT-2016-p.191-197
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/4932
dc.languageeng
dc.publisherIndonesian Center for Animal Science Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/proceedings/article/view/1479/1352
dc.rightsCopyright (c) 2017 Proceedings International Seminar on Livestock Production and Veterinary Technologyen-US
dc.sourceProceedings of International Seminar on Livestock Production and Veterinary Technology; 2016; 191-197en-US
dc.subjectTrypanosoma evansi; Internal Transcriber Spacer-1; PCRen-US
dc.titleApplication of Dried Blood Sample on Whatman Filter Paper for Detection of Trypanosoma evansi from Cattle in Central Kalimantan by Internal Trascriber Spacer-1 Polymerase Chain Reactionen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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