Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull

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dc.contributoren-US
dc.creatorRasad, Siti Darodjah; ANIMAL HUSBANDRY FACULTY UNIVERSITAS PADJADJARAN
dc.creatorSolihati, Nurcholidah; 1Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor, Sumedang
dc.creatorWinangun, Kikin; Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor, Sumedang
dc.creatorYusrina, Annisa; Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor, Sumedang
dc.creatorAvicenna, Fahmy; Animal Husbandry Faculty, Universitas Padjadjaran, Jl. Raya Bandung Sumedang KM 21, Jatinangor, Sumedang
dc.date2020-09-02
dc.date.accessioned2020-11-06T02:28:28Z
dc.date.available2020-11-06T02:28:28Z
dc.descriptionThe research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA).  A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test.  Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P<0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm.  The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%.  Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%.  It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm.en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2494
dc.identifier10.14334/jitv.v25i3.2494
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/10427
dc.languageeng
dc.publisherIndonesian Center for Animal Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2494/2169
dc.rightsCopyright (c) 2020 Jurnal Ilmu Ternak dan Veterineren-US
dc.sourceJurnal Ilmu Ternak dan Veteriner; Vol 25, No 3 (2020): SEPTEMBER 2020; 112-119en-US
dc.source2252-696X
dc.source0853-7380
dc.subjectIncubation Time, Pasundan Bull, Sexed Spermen-US
dc.titleEffect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bullen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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