Teknik Penyimpanan dan Pemeliharaan Mikroba
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Date
2001
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Balai Penelitian Bioteknologi Tanaman Pangan
Abstract
Techniques for Conservation and Storage of Microbes. Muhammad Machmud. Microbiologists need to keep their microbial culture collections for different purposes. Therefore, they have to conserve and store their collections in order to maintain survival and genetic stability of the microbes. In this paper, the term microbes is synonymy to microorganisms including viruses, bacteria, fungi, nematodes, yeast, algae, and protozoa, that are saprophytic, epiphytic, parasitic, antagonistic, and pathogenic. Based on the period and objective, the microbial conservation and storage were distinguished into (1) short-term, for a short period of time, such as from isolation until correct identification is done; (2) intermediate-term, such as the duration of a research project, and (3) long-term, for collection, conservation, or research references. The life duration of a microbial isolate is affected by several factors such as the microbial characters, composition, and pH of the medium, aeration, relative humidity, and temperature of
the storage. Therefore, there are various different techniques for conservation and storage of the microbiaf cultures. Generally, the microbiologists agree that the preferred techniques for long-term conservation and storage of microbes are freeze drying or lyophilization technique and cryogenic technique. However, not all laboratory are accessible to facilities for those tech niques. Alternative techniques need to be used without reducing the success of the objective of the microbial conservation and storage. This paper is a brief review of general techniques for conservation and storage of microbial cultures with more emphasize on bacteria.
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Keywords
Conservation, storage, microbes