Exopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatory

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dc.contributoren-US
dc.creator., Kusmiati; Pusat Penelitian Bioteknologi LIPI
dc.creatorAfiati, Fifi; Pusat Penelitian Bioteknologi LIPI
dc.creatorKukihi, Farha Elein; Institut Sain dan Teknologi Nasional
dc.date2017-09-26
dc.date.accessioned2018-06-04T06:46:45Z
dc.date.available2018-06-04T06:46:45Z
dc.date.issued2017-09-26
dc.descriptionImmunomodulatory activity assay and characterization of exopolysaccharide (EPS) from Lactic Acid Bacteria (LAB) was done in Bogor. Bacteria used in this study was LAB strains of Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Exopolysaccharide was extracted from L. delbrueckii subsp. bulgaricus and S. thermophilus then characterized with FT-IR spectrophotometer to determine the functional group. IR spectrum analysis using Fourier Transform-Infra Red (FT-IR) showed that EPS from both LAB isolates were carbohydrate compounds. Immunomodulatory activity in vivo from EPS was measured using phagocytic activity and phagocytic capacity macrophage cells from mice peritoneal cavity fluid. Exopolysaccharide were given orally to mice in concentrations of 100 μg/ml, 200 μg/ml and 300 μg/ml for 14 days then the mice were infected with Staphylococcus aureus. Result showed that EPS from both LAB isolate enhanced either phagocytic activity and phagocytic capacity macrophage cell from mice peritoneal fluid. EPS from L. delbrueckii subsp. bulgaricus concentration 300 μg/ml showed the highest phagocytic activity of macrophage cells and EPS from S. thermophilus concentration 300 μg/ml showed the highest phagocytic capacity. It is concluded that EPS potency tested as immunomodulatory derived from a culture of L. delbrueckii and S. thermophilus subsp.bulgaricus are able to increase the activity and phagocytosis murine peritoneal macrophages.en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1414
dc.identifier10.14334/jitv.v21i3.1414
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2579
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1414/pdf-
dc.rightsCopyright (c) 2017 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 21, No 3 (2016): SEPTEMBER 2016; 182-189en-US
dc.titleExopolysaccharide (EPS) activity test of lactic acid bacteria (LAB) as immunomodulatoryen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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