Aplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Province

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dc.creatorSumantri, Cece
dc.creatorDiyono, R
dc.creatorFarajallah, A
dc.creatorAnggraeni, A
dc.creatorAndreas, E
dc.date2012-03-04
dc.date.accessioned2018-06-04T06:48:31Z
dc.date.available2018-06-04T06:48:31Z
dc.date.issued2012-03-04
dc.descriptionSelection using genetic markers are commonly performed to improve livestock productivity in the livestock industry. The objectives of this study were to identify growth hormone genes family (GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI) polymorphisms of Banten buffalo population consisted of Pandeglang and Lebak subpopulations. A total number of 209 blood samples were collected from 15 districts. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products of GH, GHR, GHRH and Pit-1 Genes were digested with MspI, AluI, HaeIII and HinfI enzyme restriction. Fragments of GH|MspI, GH|AluI, GHR|AluI, GHRH|HaeIII and Pit-1|HinfI were detected by EtBr method. The results showed that GH|MspI and GHRH|HaeIII loci were polymorphic, GH|AluI, GHR|AluI and Pit-1|HinfI, loci were monomorphic. GH allele (-) at locus GH|MspI was only found in Cisata (0.03) and Menes (0.11). Allele B at locus GHRH|HaeIII only found in Cibadak (0.42), Cisata (0.30) and Menes (0.11). In the total population of Banten locus GH|MspI have low diversity (He = 0.02) and polymorphic information content (Pic = 0.02), whereas GHRH|HaeIII locus has a higher diversity (He = 0.23) and Pic (0.22). Key Words: Polymorphism, Growth Hormone Genes, Buffaloen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/668
dc.identifier10.14334/jitv.v15i4.668
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3360
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/668/677
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 15, No 4 (2010): DECEMBER 2010; p.286-296en-US
dc.titleAplication of growth hormone genes familly (GH, GHR, GHRH and Pit-1) for detecting genetic variation of buffaloes in Pandeglang and Lebak districts in Banten Provinceen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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