Effect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents.

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dc.creatorOS, Ariantie; Mahasiswa Program Studi Biologi Reproduksi, Sekolah Pascasarjana, Institut Pertanian Bogor, Jln. Agatis Kampus IPB Darmaga Bogor 16680,
dc.creatorTL, Yusuf
dc.creatorD, Sajuthi
dc.creatorRI, Arifiantini
dc.date2014-02-14
dc.date.accessioned2018-06-04T06:47:53Z
dc.date.available2018-06-04T06:47:53Z
dc.date.issued2014-02-14
dc.descriptionA cryoprotectan is component that must be present in a cryopreservation medium to minimize the physical and chemical stresses resulting from the cooling, freezing and thawing of sperm cells. This study was carried out to determine the effect of glycerol (G) and dimethylformamide (DMF) as cryprotective agent in tris-egg yolk (TEY) trehalose (T) and tris-soya (TS) raffinose (R) diluents. Semen were collected from three sexually mature bucks using artificial vagina, evaluated and divided into four aliquot. Each of them was diluted with TEY suplemented with 50 mM trehalose and TS supplemented with 50 mM raffinose, added with glycerol or DMF 4% (v/v). Diluted semen was packed in minitube straw (100 x 106 sperm/0.25 mL) and equilibrated for 4 hours at 5°C, then freeze in N2 vapor for 10 minutes in styrofoam box and stored in liquid N2 container (-196) until futher evaluation. Progressive motility, viability and plasma membrane intact were evaluated after thawed at 37°C for 30 seconds factorial experimental design (2 x 2) was used in this study. The sperm motility in TEYTG was significantly higher (65.07±5.38%) compared to TEYTDMF (61.67±5.55%). In contrast sperm diluted with TSRDMF indicated better motility (42.22±8.13%) than TSRG (39.07±5.38%). It was concluded that cryoprotectant had different effect on different diluents. Key Words: Buck Etawah Crossbreed, Cryoprotectant, Diluent, Frozen Semenen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/327
dc.identifier10.14334/jitv.v18i4.327
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3071
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/327/336
dc.rightsCopyright (c) 2014 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 18, No 4 (2013): DECEMBER 2013; p.239-250en-US
dc.titleEffect of glycerol and dimethylformamide cryoprotectants on buck Etawah Crossbreed frozen semen using modified tris diluents.en-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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