Growth response of leucaena embryogenic callus on embryo age differences and Auxin 2,4-Dichlorophenoxyacetic acid

dc.contributorPrime Research according to the mandate Division” directorate general of higher education 2015en-US
dc.creatorManpaki, Satria Julier; Graduate School of Bogor Agricultural University
dc.creatorPrihantoro, Iwan; IPB
dc.creatorKarti, Panca Dewi M.H.; IPB
dc.date2018-05-25
dc.descriptionLeucaena (Leucaena leucocephala cv. Tarramba) is a source of protein from the legume family. Tarramba varieties able to adapt well in tropical area such as West Nusa Tenggara and East Nusa Tenggara. This study aimed to identify the growth response and embryogenic callus morphology of lamtoro (L. leucaena cv. Tarramba) in embryos different ages and auxin 2,4-D levels. This research was used explants derived from lamtoro (L. leucocephala cv. Tarramba) as much 400 explants. this study conducted of 16 treatments with two factors, the first factor is the provision of PGR 2,4-D concentration of 0.5 mg L-1, 1 mg L-1, 1.5 mg L-1 and 2 mg L-1. The second factor was the type of embryo such as mature embryo, cotyledon, heart, and pre-globular. Each treatment was repeated 25 replications. This study uses a randomized complete design (CRD) with two factor. Data were analyzed using analysis of variance and if there was significant difference, data were further analyzed using Duncan’s multiple range test. Analysis of variance showed that PGR 2,4-D at a concentration of 1.5 mg L-1 and the type of optimum embryo was mature embryo callus on parameters as height, diameter increment callus, and callus color scores were significantly different (P <0.05). callus texture parameter indicates results that are uniform throughout the treatment that was compact callus. Lamtoro plant embryogenic callus (L. leucocephala cv. Tarramba) indicate an optimal response at the concentration of PGR 2,4-D 1.5 mg L-1 and the type of embryo was mature embryo. en-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1538
dc.identifier10.14334/jitv.v23i2.1538
dc.languageeng
dc.publisherIndonesian Center for Animal Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1538/pdf%3D
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/1538/42
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/downloadSuppFile/1538/43
dc.rightsCopyright (c) 2018 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.sourceJurnal Ilmu Ternak dan Veteriner; Vol 23, No 2 (2018): JUNE 2018; 95-102en-US
dc.source2252-696X
dc.source0853-7380
dc.subjectAuxin 2,4-D, Callus, Embryo, Embryogenic, Lamtoro cv. Tarrambaen-US
dc.titleGrowth response of leucaena embryogenic callus on embryo age differences and Auxin 2,4-Dichlorophenoxyacetic aciden-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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