Isolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnya

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dc.contributoren-US
dc.creatorRichana, Nur; Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian, Jl. Tentara Pelajar 12, Bogor 16114
dc.creatorIrawadi, Tun T.; Jurusan Kimia Fakultas MIPA, Institut Pertanian Bogor, Kampus IPB Baranangsiang, Jl. Pajajaran, Bogor 16144
dc.creatorNur, Anwar; Jurusan Kimia Fakultas MIPA, Institut Pertanian Bogor, Kampus IPB Baranangsiang, Jl. Pajajaran, Bogor 16144
dc.creatorSyamsu, Khaswar; Jurusan Teknologi Industri Pertanian, Fakultas Teknologi Pertanian, Institut Pertanian Bogor, Kampus Darmaga, Bogor 16680
dc.date2016-08-09
dc.date.accessioned2018-05-02T06:15:00Z
dc.date.available2018-05-02T06:15:00Z
dc.date.issued2016-08-09
dc.descriptionXylanase is an extracellular enzyme produced bymicroorganisms. This enzyme is able to hydrolise xylane(hemicellulose) to produce xylooligosaccharide and xylose.Thermoalkaliphilic xylanase is an agent that can be used asa substitute in the pulp whitening process instead of chlorine.A study was done to isolate, identificate of bacteria andcharacterize xylanase. The isolation of xylanase producingbacteria has been done from soil and waste of starch industry.Colonies which produced clearing zone were presumedas xylanolytic bacteria and chosen for further screening.Identification of potential isolate in xylanase production wasdone using 16S ribosomal RNA sequencing. Isolate Bacilluspumilus RXA-III5 originated from lime or alkaline soil wasmore potential isolate in xylanase production than other 24isolates. Precipitation of xylanase, that was done usingammonium sulphate followed by dialyzes produced xylanaseof a higher specific activity (267.1 U.mg-1) than that usingacetone (131.1 U.mg-1) and ethanol (186.65 U.mg-1). Xylanasewas done at purification produced three fractions of xylanase.Xylanase characteristics consist of pH and temperature(9 and 50oC), Km and Vmaks value 6 mg.ml-1 and 0.2mol.minute-1, respectively. The Fe2+ was the strongest activetorand Mg2+ was the strongest inhibitor activity. This enzymewas detected as a cellulose-free xylanase. Xylanase is aprospective agent for bio-bleaching of paper.en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3788
dc.identifier10.21082/jbio.v4n1.2008.p24-34
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/469
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3788/3137
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.source2549-1547
dc.source1907-1094
dc.sourceJurnal AgroBiogen; Vol 4, No 1 (2008): April; 24-34en-US
dc.titleIsolasi Identifikasi Bakteri Penghasil Xilanase serta Karakterisasi Enzimnyaen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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