Response of Anther Donor Genotypes (F1) from Indica x Indica Crosses to Rice Anther Culture
dc.contributor | en-US | |
dc.creator | Dewi, Iswari Saraswati; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Jl. Tentara Pelajar 3A, Bogor 16111,West Java-Indonesia | |
dc.creator | Putri, Nuha Hera; Department of Agronomy and Horticulture, Bogor Agricultural University, Jl. Meranti, Darmaga 16680, Bogor, West Java-Indonesia. | |
dc.creator | Purwoko, Bambang Sapta; Department of Agronomy and Horticulture, Bogor Agricultural University, Jl. Meranti, Darmaga 16680, Bogor, West Java-Indonesia. | |
dc.date | 2019-09-03 | |
dc.description | Anther culture is one of the in vitro techniques that can be used to accelerate the obtainment of purelines in the form of dihaploid (DH) plants resulted from androgenesis. The objectives of this research were to study response of anther donor genotypes in rice anther culture of F1 populations derived from indica x indica crosses and obtain spontaneous DH lines to be used further for variety development program. Anther donor plant used for the experiment consisted of four kinds of F1 populations derived from single crosses, i.e. IR85/I-5, BioR-81/I-5, Bio-R81/O18, and BioR-82/O-18. The result indicated that kindy of all F1 plants showed similar response to callus induction, but significatly different responses to plant regeneration. Genotypes of anther donor plants (F1) significantly affected the percentage of calli producing green plantlet, but not significantly affected the percentage of calli producing albino plantlet. Anther culturability of anther donor plants (F1) was relatively high, ranged from 2.0 to 7.0%. A total of 73 first generation of spontaneous DH lines (38.8%) from the grew out green plantlets was obtained from this study. The resulted DH lines are readily available to be selected and be used in rice cultivar development program. | en-US |
dc.format | application/pdf | |
dc.identifier | http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/9047 | |
dc.identifier | 10.21082/jbio.v15n1.2019.p45-52 | |
dc.language | eng | |
dc.publisher | Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian | en-US |
dc.relation | http://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/9047/8542 | |
dc.rights | Copyright (c) 2019 Jurnal AgroBiogen | en-US |
dc.rights | http://creativecommons.org/licenses/by-nc-sa/4.0 | en-US |
dc.source | Jurnal AgroBiogen; Vol 15, No 1 (2019): June; 45-52 | en-US |
dc.source | 2549-1547 | |
dc.source | 1907-1094 | |
dc.subject | Androgenesis; dihaploid; indica x indica; padi. | en-US |
dc.title | Response of Anther Donor Genotypes (F1) from Indica x Indica Crosses to Rice Anther Culture | en-US |
dc.type | info:eu-repo/semantics/article | |
dc.type | info:eu-repo/semantics/publishedVersion | |
dc.type | Peer-reviewed Article | en-US |