INHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORS

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dc.contributoren-US
dc.creatorRiyanti, Eny Ida; Indonesian Centre Agricultural Biotechnology and Genetic Resources Research and Development Jl. TentaraPelajar No. 3A.Bogor 16111, West Java, Indonesia
dc.creatorListanto, Edy; Indonesian Centre Agricultural Biotechnology and Genetic Resources Research and Development Jl. TentaraPelajar No. 3A.Bogor 16111, West Java, Indonesia
dc.date2017-09-14
dc.date.accessioned2018-05-02T01:18:08Z
dc.date.available2018-05-02T01:18:08Z
dc.date.issued2017-09-14
dc.descriptionBiomass from lignocellulosic wastes is a potential source for biobased products.  However, one of the constraints in utilization of biomass hydrolysate is the presence of inhibitors. Therefore, the use of inhibitor-tolerant microorganisms in the fermentation is required. The study aimed to investigate the effect of a mixture of inhibitors on the growth of Saccharomyces cerevisiae strain I136 grown in medium containing synthetic inhibitors (acetic acid, formic acid, furfural, 5-hydroxymethyl furfural/5-HMF, and levulinic acid) in four different concentrations with a mixture of carbon sources, glucose  (50 g.l-1) and xylose (50 g.l-1) at 30oC. The parameters related to growth and fermentation products were observed. Results showed that the strain was able to grow in media containing natural inhibitors (BSL medium) with µmax of 0.020/h. Higher level of synthetic inhibitors prolonged the lag phase, decreased the cell biomass and ethanol production, and specific growth rate. The strain could detoxify furfural and 5-HMF and produced the highest ethanol (Y(p/s) of 0.32 g.g-1) when grown in BSL. Glucose was utilized as its level decreased in a result of increase in cell biomass, in contrast to xylose which was not consumed. The highest cell biomass was produced in YNB with Y (x/s) value of 0.25 g.g-1. The strain produced acetic acid as a dominant side product and could convert furfural into a less toxic compound, hydroxyl furfural. This robust tolerant strain provides basic information on resistance mechanism and would be useful for bio-based cell factory using lignocellulosic materials. en-US
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dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/3629
dc.identifier10.21082/ijas.v18n1.2017.p17-24
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/54
dc.languageeng
dc.publisherIndonesian Agency for Agricultural Research and Developmenten-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/3629/6671
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3629/506
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/downloadSuppFile/3629/507
dc.rightsCopyright (c) 2017 Indonesian Journal of Agricultural Scienceen-US
dc.rightshttp://creativecommons.org/licenses/by-sa/4.0en-US
dc.source2354-8509
dc.source1411-982X
dc.sourceIndonesian Journal of Agricultural Science; Vol 18, No 1 (2017): June 2017; 17-24en-US
dc.titleINHIBITION OF THE GROWTH OF TOLERANT YEAST Saccharomyces cerevisiae STRAIN I136 BY A MIXTURE OF SYNTHETIC INHIBITORSen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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