Association of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattle

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dc.contributorIPB University, Department of Animal Production and Technology, Faculty of Animal Scienceen-US
dc.creatorDairoh, Dairoh; IPB University
dc.creatorJakaria, Jakaria; IPB University
dc.creatorUlum, Mokhamad Fakhrul; IPB University
dc.creatorIshak, Andi Baso Lompengeng; IRIAP
dc.creatorSumantri, Cece; IPB University
dc.date2021-06-29
dc.date.accessioned2021-09-07T02:48:12Z
dc.date.available2021-09-07T02:48:12Z
dc.descriptionBali cattle (Bos javanicus) are native Indonesian cattle, domesticated from banteng (Bibos banteng). Genes that have an important role in meat quality are calcium-activated neutral protease genes, known as calpains (CAPN). The objective of this study was to evaluate the polymophisms of calpain gene SNP g.232 G>T by PCR-RFLP technique and its influence on growth trait and meat quality of Bali cattle detected by ultrasound imaging of longissimus dorsi thickness (LDT), back fat thickness (BFT), marbling score (MS), and intramuscular fat percentage (PIMF). The polymorphisms of CAPN1 gene were analyzed by PCR-RFLP using BglII restriction enzyme (n=52 cattle). The ultrasound images of longissimus dorsi muscle were carried out transversally and longitudinal between 12th -13th thoracic vertebrae then analyzed by Image-J NIH software. Result showed that  SNP g.232 G>T of CAPN1 gene was polymorphic in Bali cattle. SNP g.232 G>T of CAPN1 gene in Bali cattle has higher diversity which was showed of 0.48 heterozygosity value and was in Hardy-Weinberg equilibrium. The polymorphisms of SNP g.232 G>T was associated significantly (P<0.05) with bodyweight at 730 days, marbling score (MS), and intramuscular fat percentage (PIMF). It suggests that the CAPN1 gene in Bali cattle is a candidate for Marker Assisted Selection (MAS), which influences body weight at 730 days, marbling score, and percentage of intramuscular fat.en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2701
dc.identifier10.14334/jitv.v26i2.2701
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/13342
dc.languageeng
dc.publisherIndonesian Center for Animal Research and Development (ICARD)en-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/2701/2288
dc.rightsCopyright (c) 2021 Jurnal Ilmu Ternak dan Veterineren-US
dc.sourceJurnal Ilmu Ternak dan Veteriner; Vol 26, No 2 (2021): June 2021; 49-56en-US
dc.source2252-696X
dc.source0853-7380
dc.subjectBali cattle, Calpain gene, Growth, Meat quality, Ultrasounden-US
dc.titleAssociation of SNP g.232 G>T Calpain Gene with Growth and Live Meat Quality Prediction using Ultrasound Images in Bali Cattleen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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