The effect of kinds and concentration of cryoprotectant and thawing methods on frozen semen of Arab chicken

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dc.creatorIskandar, Sofjan
dc.creatorMardalestari, Rufika
dc.creatorHernawati, Resmi
dc.creatorMardiah, Enok
dc.creatorWahyu, Endang
dc.date2012-02-05
dc.date.accessioned2018-06-04T06:47:14Z
dc.date.available2018-06-04T06:47:14Z
dc.date.issued2012-02-05
dc.descriptionThe success of freezing chicken semen is the hope for preserving Indonesian native chickens. Semen from twenty Arab roosters were collected using massage technique once in a week. Cryoprotectant DMA (dimethyl acetamide) or DMF (dimethyl formalmide) of 7 or 9% and thawing A at temperature of 30oC for 30 seconds or in B at 5oC for 5 minutes. The volume of fresh semen was 0.3 ± 0.072 ml/ejaculate, white colour, rather thick to thick, with 2200 ± 372 millions sperms/ml and pH 6.95 ± 0.32, 4+/3+ mass movement, 80% motility, 84 ± 4.48% and abnormality of 14.75 ± 1.28%. There were not statistically significant (P>0.05) effect of interaction of treatments (kinds and concentrations of cryoprotectant, and thawing methods) on motility and live-sperms. Sperm motility preserved with DMA (34.69%) significantly higher than with DMF (29.84%). Sperm motility was also significantly higher (34.53%) when preserved with 7% cryoprotectant than with 9% (30%). Thawing-A significantly gave higher motility (35.31%) than thawing-B did (29.22%). Live-sperms of semen preserved with DMA (46.75%) was significantly higher than with DMF (41.72%). Cryoprotectant concentration of 7% gave higher live-sperms (46.98%) than of 9% did (41.48%). Thawing-A left live-sperms of 47.14%, which was significantly higher than thawing-B did (41.30%). Key Words: Frozen Semen, Arab Rooster, Cryoprotectants, Thawing Methodsen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/505
dc.identifier10.14334/jitv.v11i1.505
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2795
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/505/514
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 11, No 1 (2006); p.34-38en-US
dc.titleThe effect of kinds and concentration of cryoprotectant and thawing methods on frozen semen of Arab chickenen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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