Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris

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dc.contributoren-US
dc.creatorHusin, Bahagiawati A.; Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
dc.date2016-08-05
dc.date.accessioned2018-05-02T06:15:01Z
dc.date.available2018-05-02T06:15:01Z
dc.date.issued2016-08-05
dc.descriptionPlant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean (Phaseolus vulgaris) contain a glycoprotein that inhibits activity of α-amylase of insects. A α-amylase inhibitor was purified from the common bean seeds. The purified α-amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus. The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α-amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects.en-US
dc.formatapplication/pdf
dc.identifierhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709
dc.identifier10.21082/jbio.v1n1.2005.p7-12
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/481
dc.languageeng
dc.publisherBalai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanianen-US
dc.relationhttp://ejurnal.litbang.pertanian.go.id/index.php/ja/article/view/3709/3085
dc.rightsCopyright (c) 2016 Jurnal AgroBiogenen-US
dc.source2549-1547
dc.source1907-1094
dc.sourceJurnal AgroBiogen; Vol 1, No 1 (2005): April; 7-12en-US
dc.titleIsolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgarisen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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