Characterisation of protein antigen from Fasciola gigantica of different age

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dc.creatorEstuningsih, S Endah
dc.creatorWidjajanti, S
dc.date2014-02-25
dc.date.accessioned2018-06-04T06:46:49Z
dc.date.available2018-06-04T06:46:49Z
dc.date.issued2014-02-25
dc.descriptionThe protein antigens extracted from adult fluke Fasciola gigantica, 3, 6 and 9 weeks old and newly excysted juvenile (NEJ) were identified using SDS-PAGE and immunoblotting techniques. Sera from fat-tailed sheep which artificially infected with the metacercariae of F. gigantica were used for immunoblotting. The results showed that the protein antigen profile of adult fluke, 6 and 9 weeks old flukes had similar. Bands with molecular weight between 24 kDa to 114 kDa. Protein bands with molecular weight <24 kDa and >198 kDa were also detected from the adult fluke. The use of immunoblotting technique, there were two antigenic protein molecules identified from adult fluke, NEJ and 3, 6, and 9 weeks old fluke with the molecular weight 46 kDa and 47 kDa. The protein band with molecular weight >198 kDa shown thicker on the NEJ than that of adult fluke, and 6 and 9 weeks old flukes. The role of protein with molecular weight of 46 and 47 kDa were the interested findings need to be evaluatedfor serological analysis.   Key words : Protein antigen, Fasciola gigantica, SDS-PAGE, immunoblottingen-US
dc.formatapplication/pdf
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/138
dc.identifier10.14334/jitv.v4i1.138
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/2618
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/138/138
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 4, No 1 (1999): MARCH 1999; p.60-64en-US
dc.titleCharacterisation of protein antigen from Fasciola gigantica of different ageen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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