Antigen detection of rabies virus in brain smear using direct Rapid Immunohistochemistry Test

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dc.contributoren-US
dc.creatorR, Damayanti
dc.creatorI, Rahmadani
dc.creatorY, Fitria
dc.date2014-04-25
dc.date.accessioned2018-06-04T06:48:27Z
dc.date.available2018-06-04T06:48:27Z
dc.date.issued2014-04-25
dc.descriptionRabies is zoonotic disease caused by a fatal, neurotropic virus. Rabies virus is classified into the Genus of Lyssavirus under the yang family of Rhabdoviridae. Rabies affecting hot- blooded animals, as well as human. Dogs, cats, monkeys are the vectors or reservoirs for rabies and the virus was transmitted through the saliva after infected animal’s bites. The aim of this study was to conduct rapid diagnosis to detect rabies viral antigen in brain smear using immunohistochemical (IHC) method namely direct Rapid Immunohistochemical Test (dRIT). A total number of 119 brain samples were achieved from Bukittinggi Veterinary Laboratory, West Sumatra. Standardisation and validation of the method were compared to Fluorescent Antibody Test (FAT) as a golden standard for rabies diagnosis. Results show that dRIT was a very good method, it can be performed within two hours without the need of fluorescent microscope. The samples were tested using FAT and from 119 samples tested, 80 (67.23%) samples were positive for rabies and 39 (32.77%) samples were negative for rabies whereas using dRIT showed that 78 (65.54%) samples were positive for rabies and 41 (34.45%) samples were negative for rabies. The dRIT results were validated by comparing them with FAT results as a golden standard for rabies. The relative sensitivity of dRIT to FAT was 97.5% and the relative specificity to FAT was 100% (with Kappa value of 0.976, stated as excellent). The achievement showed that dRIT is very potential diagnostic tool and is highly recommended to be used widely as a rapid diagnosis tool for rabies. Key Words: Rabies, Antigen Detection, Immunohistochemistry, Direct Rapid Immunohistochemical Test (dRIT)en-US
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dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/994
dc.identifier10.14334/jitv.v19i1.994
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3323
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/994/pdf
dc.rightsCopyright (c) 1970 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 19, No 1 (2014): MARCH 2014; p.52-58en-US
dc.titleAntigen detection of rabies virus in brain smear using direct Rapid Immunohistochemistry Testen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
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