Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol

Simple item page
dc.contributoren-US
dc.creatorKusumaningrum, Diana Andrianita; IRIAP
dc.creatorPurwantara, Bambang; IPB
dc.creatorYusuf, Tuti L.; IPB
dc.creatorSitumorang, Polmer; IRIAP
dc.date2015-12-01
dc.date.accessioned2018-06-04T06:48:25Z
dc.date.available2018-06-04T06:48:25Z
dc.date.issued2015-12-01
dc.descriptionCryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media.  The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Timeen-US
dc.formatapplication/pdf
dc.formattext/html
dc.identifierhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1240
dc.identifier10.14334/jitv.v20i4.1240
dc.identifier.urihttps://repository.pertanian.go.id/handle/123456789/3311
dc.languageeng
dc.publisherIndonesian Animal Sciences Societyen-US
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1240/pdf
dc.relationhttp://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/1240/1292
dc.rightsCopyright (c) 2015 Indonesian Journal of Animal and Veterinary Sciencesen-US
dc.rightshttp://creativecommons.org/licenses/by/4.0en-US
dc.source2252-696X
dc.source0853-7380
dc.sourceIndonesian Journal of Animal and Veterinary Sciences; Vol 20, No 4 (2015): DECEMBER 2015; 233-241en-US
dc.titleMicroencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerolen-US
dc.typeinfo:eu-repo/semantics/article
dc.typeinfo:eu-repo/semantics/publishedVersion
dc.typePeer-reviewed Articleen-US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Microencapsulation of bovine spermatozoa- Cryopreservation of microencapsulation sperm using glycerol.pdf
Size:
361.46 KB
Format:
Adobe Portable Document Format
Description:
Download
License bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
license.txt
Size:
0 B
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Jurnal Ilmu Ternak dan Veteriner