Optimasi Metode Cryotherapy untuk Mengeliminasi Virus pada Tunas Kentang In Vitro (Optimation of Cryotherapy Method to Eliminate Virus on In Vitro Potato Shoot Tips)

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dc.contributor en-US
dc.creator Astarini, Ida Ayu; Program Studi Biologi FMIPA, Universitas Udayana, Kampus Bukit Jimbaran, Bali, Indonesia
dc.creator Chappell, Angel; Texas A&M University, Department of Horticultural Sciences, College Station, Texas, USA
dc.creator Scheuring, Douglas; Texas A&M University, Department of Horticultural Sciences, College Station, Texas, USA
dc.creator Thompson, Sean Michael; Texas A&M University, Department of Horticultural Sciences, College Station, Texas, USA
dc.creator Miller Jr., Julian Creighton; Texas A&M University, Department of Horticultural Sciences, College Station, Texas, USA
dc.date 2016-06-26
dc.date.accessioned 2018-05-02T06:25:51Z
dc.date.available 2018-05-02T06:25:51Z
dc.date.issued 2016-06-26
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/3555
dc.identifier 10.21082/jhort.v26n1.2016.p97-102
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/997
dc.description Penggunaan benih kentang generasi awal dan bebas virus merupakan kunci keberhasilan produksi kentang berkualitas. Cryotherapy (perendaman dalam nitrogen cair) merupakan teknik terbaru untuk mengeliminasi virus pada benih kentang. Salah satu kendala dalam penerapan teknologi cryotherapy ialah tingkat daya hidup eksplan yang masih rendah. Penelitian ini bertujuan untuk mengetahui efektifitas teknik enkapsulasi-dehidrasi untuk mendapatkan tunas yang sehat setelah perendaman dalam nitrogen cair. Ujung tunas in vitro ukuran 2–3 mm dari empat genotipe kentang di prakultur selama 3 hari secara bertahap pada media MS dengan penambahan gula 0,25 M, 0,5 M, dan 0, 75 M. Kemudian tunas dienkapsulasi, didehidrasi selama 5 jam, lalu direndam dalam nitrogen cair selama 60 menit lalu dihangatkan kembali dalam waterbath selama 3 menit. Tunas dalam kapsul kemudian dikulturkan pada media MS +30 g/l sukrosa + 8 g/l agar + 0,4 mg/l BAP + 1 mg/l GA3 untuk pemulihan, lalu dipelihara di ruang kultur dengan suhu 24oC. Daya hidup ujung tunas diamati pada minggu ke-8 dengan menggunakan kriteria skoring sebagai berikut: (1) pemutihan jaringan dan tidak ada respons pertumbuhan, (2) kalus mencokelat, (3) kalus hijau, (4) tumbuh tunas, dan (5) planlet sehat. Hasil penelitian menunjukkan daya hidup ujung tunas bervariasi antargenotipe. Skor daya hidup berkisar 1–2 (frekuensi 2–10) pada perlakuan nitrogen cair, yang menunjukkan tidak ada respons pertumbuhan tunas, beberapa memperlihatkan pertumbuhan kalus. Tunas pada perlakuan kontrol (tanpa perendaman dalam nitrogen cair) menunjukkan skor daya hidup 5 (frekuensi 1–7), di mana ujung tunas mampu beregenerasi menjadi planlet.KeywordsCryopreservation; Solanum tuberosum; Kultur jaringan tanaman; Eliminasi virusAbstractVirus-free, early generation seed is a key in the production of high quality potatoes. Cryotherapy (exposure to liquid nitrogen) is a new and promising method of virus elimination. One bottleneck in cryotheraphy method is survival of the explants after treatment with liquid nitrogen. This study investigated the effectiveness of enkapsulasi-dehidrasi method to obtain survival explants. Shoot tips were precultured for 3 days in MS media with sucrose addition of 0.25 M, 0.5 M and 0.75 M. Shoot tips were then encapsulate, dehydrate for 5 hours, expose to liquid nitrogen for 60 minutes and rewarm in waterbath for 3 minutes. Beads with shoot tips were then cultured in MS media + 30 g/l sucrose + 8 g/l agar + 0.4 mg/l BAP + 1 mg/l GA3 for recovery, and placed in 24oC culture room. Shoot tip survival was assessed at 8 weeks using the following scoring criteria: (1) tissue bleaching and no growth response, (2) brown callus, (3) green callus, (4) shoot growth, and (5) plantlet establishment. Survival was varied among genotypes. Survival scored between 1–2 (frequency 2–10) on liquid nitrogen treatment, showing shoot tips are mostly has no growth response, only some callus growth. Shoot tips on control treatment (without exposure in liquid nitrogen) shows survival scored 5 (frequency 1–7), i.e. shoot tips able to regenerate into plantlets. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Horticulture Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/3555/3008
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/downloadSuppFile/3555/525
dc.rights Copyright (c) 2016 Indonesian Center for Horticulture Research and Development en-US
dc.rights http://creativecommons.org/licenses/by-sa/4.0 en-US
dc.source 2502-5120
dc.source 0853-7097
dc.source Jurnal Hortikultura; Vol 26, No 1 (2016): Juni 2016; 97-102 en-US
dc.title Optimasi Metode Cryotherapy untuk Mengeliminasi Virus pada Tunas Kentang In Vitro (Optimation of Cryotherapy Method to Eliminate Virus on In Vitro Potato Shoot Tips) en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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