CONSTRUCTION, EXPRESSION AND PURIFICATION OF RECOMBINANT PRE-MATURE PEPTIDE OF PLANTARICIN F FROM Lactobacillus plantarum S34 IN Escherichia coli

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dc.contributor en-US
dc.creator Kusdianawati, Kusdianawati; School of Biotechnology, Bogor Agricultural University Jalan Raya Darmaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia
dc.creator Mustopa, Apon Zaenal; Research Center for Biotechnology, Indonesian Institute of Sciences Jalan Raya Bogor km 46, Cibinong 16911, Bogor, Indonesia
dc.creator Suharsono, Suharsono; School of Biotechnology, Bogor Agricultural University Jalan Raya Darmaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia
dc.creator Budiarto, Bugi Ratno; Research Center for Biotechnology, Indonesian Institute of Sciences Jalan Raya Bogor km 46, Cibinong 16911, Bogor, Indonesia
dc.creator Fatimah, Fatimah; Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Jalan Tentara Pelajar No. 3A, Bogor 16111, Indonesia
dc.creator Danuri, Hasim; Department of Biochemistry, Bogor Agricultural University Jalan Agatis Kampus IPB Darmaga Bogor 16680, Indonesia
dc.date 2015-09-02
dc.date.accessioned 2018-05-02T01:18:11Z
dc.date.available 2018-05-02T01:18:11Z
dc.date.issued 2015-09-02
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/2412
dc.identifier 10.21082/ijas.v16n1.2015.p31-38
dc.identifier.uri http://repository.pertanian.go.id/handle/123456789/79
dc.description Plantaricin is one of bacteriocins that have the potential to be used as food preservative. Plantaricin is safe for human consumption because it can be easily degraded by proteolytic enzymes. The objective of this study was to express and purify recombinant pre-mature peptide of plantaricin F from Lactobacillus plantarum S34 in Escherichia coli. Plantaricin gene-specific primer was used to obtain pln F structural gene amplicon from L. plantarum S34. This amplicon was cloned in pET32a vector and expressed in E. coli BL21 (DE3) pLysS. Pre-mature plantaricin F peptide was expressed as Histagged-fusion protein and separated by Co2+-chelating affinity chromatography. L. plantarum S34-derived pre-mature plantaricin F peptide fused with thioredoxin-(His)6tag had successfully been expressed in E. coli BL21 (DE3) pLysS using pET32a as an expression vector. The fused recombinant pln F as pre-mature state expressed had a molecular mass of +24 kDa, meanwhile the fused recombinant that contained only the leader peptide of pln F appeared as +20 kDa based on SDS-PAGE separations. The optimal production of fused recombinant pln F as soluble fraction was obtained when culture condition was added with 0.5 mM of IPTG and incubated at 22°C for 5 hours (OD~1). Furthermore, the expression of fused recombinant pln F as its pre-mature peptide pointed out that the pln F’s leader peptide could be proteolytically cleaved by a system in heterologous cells. Overall, heterologous pln F production as pre-mature peptide fused with thioredoxin-(His)6tag had been well established. From this research, we expect plantaricin F can be expressed and purified in E. coli. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Agency for Agricultural Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/ijas/article/view/2412/2094
dc.rights Copyright (c) 2015 Indonesian Journal of Agricultural Science en-US
dc.rights http://creativecommons.org/licenses/by-sa/4.0 en-US
dc.source 2354-8509
dc.source 1411-982X
dc.source Indonesian Journal of Agricultural Science; Vol 16, No 1 (2015): April 2015; 31-38 en-US
dc.title CONSTRUCTION, EXPRESSION AND PURIFICATION OF RECOMBINANT PRE-MATURE PEPTIDE OF PLANTARICIN F FROM Lactobacillus plantarum S34 IN Escherichia coli en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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