Identifikasi Variabilitas Genetik Wani Bali (Mangifera caesia Jack.) dengan Analisis Penanda RAPD

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dc.contributor en-US
dc.creator Rai, I Nyoman
dc.creator Wijana, G
dc.creator Semarajaya, C G A
dc.date 2008-06-30
dc.date.accessioned 2019-10-09T09:43:38Z
dc.date.available 2019-10-09T09:43:38Z
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/806
dc.identifier 10.21082/jhort.v18n2.2008.p%p
dc.identifier.uri http://124.81.126.59/handle/123456789/7969
dc.description ABSTRAK. Wani Bali (Mangifera caesia Jack.) merupakan salah satu tanaman buah-buahan tropika tergolong kerabat mangga. Citarasa Wani Bali disukai konsumen karena daging buahnya memiliki aroma khas, rasanya manis, enak, dan daging buahnya tebal. Terdapat banyak kultivar Wani Bali dengan sifat spesifik buahnya masing-masing, tetapi secara genetik belum diketahui variabilitasnya. Penelitian ini betujuan mengidentifikasi variabilitas genetik Wani Bali dengan analisis penanda random amplified polymorphic DNA (RAPD). Penelitian dilakukan mulai bulan Februari sampai Desember 2006, berlokasi di seluruh sentra produksi Wani Bali di Bali. Pelaksanaan terdiri atas 3 tahap, yaitu (1) survei kultivar dilanjutkan dengan identifikasi karakter daun, bunga, dan buah, (2) pengumpulan sampel untuk analisis RAPD (biji dari kultivar yang telah diidentifikasi ditanam dalam polibag di rumah plastik, setelah bibit berumur 6 bulan, 5-6 lembar daunnya dipanen untuk sampel), dan (3) analisis penanda RAPD, dilakukan di Laboratorium Biomolekuler dan Immunologi, Unit Penelitian Bioteknologi Perkebunan, Bogor. Berdasarkan karakter buahnya (bentuk, rasa, ukuran, dan warna kulit) teridentifikasi 22 kultivar Wani Bali, tetapi kultivar-kultivar tersebut tidak dapat dibedakan satu dengan yang lainnya berdasarkan habitus pohon, sifat percabangan, serta karakter daun dan bunga. Variabilitas genetik Wani Bali dianalisis dengan RAPD dengan keanekaragaman mencapai 43% terdiri atas 3 kelompok. Satu-satunya kultivar yang secara genetik sangat berbeda dengan yang lainnya adalah Wani Bali Ngumpen (kultivar tanpa biji) ditemukan di Desa Bebetin, Kabupaten Buleleng. Kultivar-kultivar yang berasal dari kabupaten yang sama dan atau pada 2 kabupaten yang berdekatan mengelompok pada kelompok yang sama, kecuali Wani Bali Ngumpen asal Desa Bebetin, Buleleng.ABSTRACT. Rai, I.N., G. Wijana, and C. G. A. Semarajaya. 2008. Identification of Genetic Variability of Wani Bali (Mangifera caesia Jack.) Using RAPD Analysis Marker. Wani Bali is one of tropical fruit which belongs to genus mangifera. Consumer prefers the fruit due to the specific flavor, sweet and delicious taste, and the thickness of edible pulp. There are many cultivars of Wani Bali with specific character. However, genetic variability has not been specified. The research was aimed to identify the genetic variability by random amplified polymorphic DNA (RAPD) marker. The research was conducted from February to December 2006, located at centrals of Wani Bali in Bali. It consisted of 3 steps (1) surveying of cultivars and identification of their leaf, flower, and fruit characters, (2) collecting sample for RAPD analysis (seed of identified cultivars grown in polybag at plastichouse and after 6 months seedling, 5-6 leaves were collected as sample), and (3) analyzing RAPD, which was conducted at Biomolecular and Immunology Laboratory, Research Unit of Plantation Biotechnology, Bogor. The results revealed that according to the fruit character (shape, taste, size, and skin color of fruit) had been identified 22 cultivars, but among cultivars could not be specified by plant shape, branch type, leaf and flower characters. There are 3 groups at 43% variability according to genetic variability of Wani Bali which was analyzed by RAPD. The sole cultivar genetically significantly different among the cultivars is Wani Bali Ngumpen (seedles cultivar) from Bebetin, Buleleng District. The cultivars that were planted at the same regency and/or at 2 neighbouring regencies genetically were clustered in 1 group, excluding Wani Bali Ngumpen from Bebetin, Buleleng District. en-US
dc.format application/pdf
dc.language eng
dc.publisher Indonesian Center for Horticulture Research and Development en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/jhort/article/view/806/638
dc.rights Copyright (c) 2013 Indonesian Center for Horticulture Research and Development en-US
dc.rights http://creativecommons.org/licenses/by-sa/4.0 en-US
dc.source Jurnal Hortikultura; Vol 18, No 2 (2008): Juni 2008 en-US
dc.source 2502-5120
dc.source 0853-7097
dc.subject Mangifera caesia; Variabilitas; Genetik; RAPD. en-US
dc.title Identifikasi Variabilitas Genetik Wani Bali (Mangifera caesia Jack.) dengan Analisis Penanda RAPD en-US
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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