ANALISIS KERAGAMAN GENETIK KEDELAI INTRODUKSI MENGGUNAKAN MARKA MIKROSATELIT

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dc.contributor Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian id-ID
dc.creator Nugroho, Kristianto; BB Biogen Jalan Tentara Pelajar 3A Cimanggu Bogor
dc.creator Terryana, Rerenstradika Tizar
dc.creator Reflinur, nFN
dc.creator Asadi, nFN
dc.creator Lestari, Puji
dc.date 2017-12-06
dc.date.accessioned 2019-10-09T09:43:34Z
dc.date.available 2019-10-09T09:43:34Z
dc.identifier http://ejurnal.litbang.pertanian.go.id/index.php/IP/article/view/7317
dc.identifier 10.21082/ip.v26n2.2017.p121-132
dc.identifier.uri http://124.81.126.59/handle/123456789/7901
dc.description Soybean (Glycine max (L.) Meriil) is an important crop next to rice and corn. The development of improved varietyare important to increase national soybean production. The introduced soybean varieties is one of genetic resourcesthat can be used to create improved soybean varieties. The aim of this study was to analyze 35 introduced soybeancultivars using 15 microsatellite markers. The research was conducted in ICABIOGRAD Molecular Biology Laboratory,in January-March 2016. PCR analysis was scored as binary data and the collected data was analyzed using NTSYS andPowerMarker. Specific morphological characters from each soybean cultivar determine the genetic diversity. Significantpositive correlations were identified among morphological characters which would be helpful to improve the desiredcharacter. The result showed that 189 alleles were detected with average of 12.6 alleles per marker. The polymorphismlevel (PIC) was 0.86 (0.76-0.95). There were 12 of total markers having PIC>0.80 indicating their robustness todiscriminating soybean cultivars. The average major allele frequency was 21% and ranges from 8% (Satt100) to 39%(Satt125). Five SSRs were able to distinguish heterozygosity which varied from 0.41 (SoyF3H) to 0.82 (Satt333). Thephylogenetic analyses showed that the 35 introduced soybean cultivars were grouped into two clusters (coefficient ofsimilarity 0.82) consisting of 13 and 22 cultivars according to each genetic background without considering its countryorigin. Both the microsatellite markers and genetic diversity information in this study could be useful to assist crossingstrategy with utilizing introduced genetic materials in future soybean breeding in Indonesia. id-ID
dc.format application/pdf
dc.language eng
dc.publisher Sekretariat Badan Penelitian dan Pengembangan Pertanian en-US
dc.relation http://ejurnal.litbang.pertanian.go.id/index.php/IP/article/view/7317/7325
dc.rights Copyright (c) 2018 Informatika Pertanian en-US
dc.rights http://creativecommons.org/licenses/by-nc-sa/4.0 en-US
dc.source Informatika Pertanian; Vol 26, No 2 (2017): Jurnal Informatika Pertanian; 121-132 en-US
dc.source 2540-9875
dc.source 0852-1743
dc.subject soybean; introduced cultivars; genetic diversity; microsatellite marker id-ID
dc.title ANALISIS KERAGAMAN GENETIK KEDELAI INTRODUKSI MENGGUNAKAN MARKA MIKROSATELIT id-ID
dc.type info:eu-repo/semantics/article
dc.type info:eu-repo/semantics/publishedVersion
dc.type Peer-reviewed Article en-US


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